Polymorphism assay

polymorphisms , particularly SNPs (Single Nucleotide Polymorphisms), are sequence variations present in DNA between individuals of the same species. Their detection and quantification allow the identification of genetic markers useful for traceability, selection, and the improvement of biological performance .

In agriculture and animal breeding, these analyses offer the possibility of identifying genes of interest associated with disease resistance, productivity, or raw material quality. In the cosmetics field, the detection of polymorphisms can be used to develop personalized approaches based on skin genetics.

Analytical methods

Polymorphism quantification relies on different complementary approaches:

• Real-time PCR (qPCR) : allows for the rapid detection of specific polymorphisms in targeted samples.
• Sanger sequencing : classic method for confirming point variations.
• NGS (Next Generation Sequencing) : offers broader coverage to identify and quantify many polymorphisms in a single analysis.
• Genotyping using specific probes : a solution suitable for large-scale studies or panels of individuals.

These techniques guarantee precision and reproducibility, while adapting to the needs of research or industrial projects.

Affected matrices

Polymorphism assays can be performed on different types of biological matrices:

• DNA extracted from plants for varietal improvement and seed certification
• Yeast and microorganism DNA for biotechnology and fermentation
• Animal DNA for genetic selection, traceability and veterinary research
• human DNA in the context of personalized cosmetics or non-medical research

Industrial and scientific applications

• Agriculture and plant breeding : identification of varieties resistant to biotic or abiotic stresses.
• Animal health and livestock : monitoring polymorphisms associated with robustness and productivity.
• Personalized cosmetics : DNA analysis to offer products adapted to the skin's genetic profile.
• Biotechnology and fermentation : monitoring yeast or bacteria strains to optimize processes.
• Genetic traceability : certification of the origin and authenticity of organic products.

Additional analyses

For a comprehensive approach, polymorphism quantification can be combined with other analyses available in the YesWeLab catalogue:

• DNA sequencing (NGS) for in-depth genetic identification.
• Measurement of methanogenic flora useful in environmental and fermentation processes.

Regulations and issues

The detection of polymorphisms is governed by quality standards, particularly in the agricultural and food sectors, to ensure compliance with traceability and labeling regulations. In the cosmetics field, it is part of an innovation approach, where genetics becomes a lever for differentiation in personalized skincare.

These analyses also contribute to basic research in genomics and population biology, providing valuable data for understanding genetic diversity.

YesWeLab expertise

YesWeLab provides industry professionals and researchers with access to its network of partner laboratories accredited to ISO 17025 and certified for molecular biology methods. Each project benefits from a customized approach, tailored to the nature of the matrix, the detection method, and regulatory requirements.

Since 2020, YesWeLab has been supporting players in agriculture, animal health, biotechnology and cosmetics in their analytical needs, by offering them a centralized digital platform to easily manage their samples and results.