Piperine dosage

Piperine is a natural alkaloid derived from black pepper ( Piper nigrum ), known for its stimulating properties and its ability to enhance the bioavailability of many nutrients and active ingredients, particularly curcumin. It plays a key role in the formulation of dietary supplements and standardized plant extracts , where its content directly influences the efficacy of the finished product.

Piperine is measured by high-performance liquid chromatography (HPLC) or liquid chromatography coupled with mass spectrometry (LC-MS) . These methods ensure precise, reproducible quantification that meets international quality standards.

Principle and operation of the method

The analysis is based on the selective extraction of piperine from plant matrices or finished formulations (pepper, dry extracts, essential oils, capsules, powders). After preparation, the sample is injected into a chromatographic system.

• In HPLC-DAD , separation is done on a C18 column, with UV detection around 340 nm, suitable for aromatic alkaloids.
• In LC-MS , detection is based on measuring the mass/charge ratio (m/z), ensuring specific identification even in complex mixtures or matrices rich in phenolic compounds.

These methods allow for the accurate determination of piperine content, the evaluation of extract purity, and the verification of conformity of standardized products.

Analytical performance and related analyses

HPLC and LC-MS techniques offer high sensitivity , high specificity and measurement stability suitable for production controls and R&D.

For a complete evaluation of pepper extracts or formulations combining bioactive compounds, this assay can be combined with other services available in the YesWeLab catalogue:

• the dosage of curcumin , in order to evaluate the piperine-curcumin synergy in formulations,
• the determination of total alkaloids,
• the dosage of total polyphenols,
• or the purity dosage , useful for guaranteeing the quality of standardized extracts.

These additional analyses make it possible to obtain a complete chemical profile and to optimize the formulation of nutraceutical products.

Applications and areas of use

Piperine assay is applicable to several sectors:

• Nutraceutical : control of piperine content in food supplements promoting nutrient absorption.
• Medicinal plants Piper nigrum extracts used in stimulant or digestive products.
• R&D and formulation : evaluation of the stability and efficacy of formulations combining piperine and other natural active ingredients.
• Quality control : verification of batch conformity and alkaloid content according to manufacturer specifications.

This analysis guarantees the traceability and scientific evaluation of products based on black pepper or derived compounds.

The advantages of the YesWeLab service

YesWeLab relies on a network of ISO 17025 and COFRAC accredited laboratories , experts in chromatographic and phytochemical analyses. Each sample is entrusted to a laboratory selected according to the matrix, the method, and the level of precision required.

Thanks to its digital platform, YesWeLab simplifies analysis management: sample submission, online tracking, and rapid results delivery. Since 2020, numerous players in the nutraceutical and phytotherapy sectors have trusted us for the standardization and quality control of their natural extracts.

For any specific request regarding piperine dosage, contact our scientific team to obtain a personalized quote and support tailored to your needs.