Phenolic dosage

Phenolic compound assays allow for the assessment of the natural antioxidant content present in a wide variety of plant, food, nutraceutical, and cosmetic matrices. These compounds play a key role in protecting against oxidative stress, ensuring product stability, and preventing lipid rancidity. Their quantification is therefore a key indicator of product quality, freshness, and functional properties.

Principle and analytical methods

Two analytical approaches are commonly used for the determination of total phenols:

• Spectrophotometry (Folin-Ciocalteu method) : This is based on the reduction of Folin's reagent by phenolic compounds, resulting in the formation of a colored complex measured at a specific wavelength. This global method makes it possible to estimate the total polyphenol content expressed in gallic acid equivalents.
• High-performance liquid chromatography (HPLC) : This technique allows the identification and quantification of each phenolic molecule individually, such as gallic acid , catechin , quercetin , rutin or caffeine . It offers superior resolution and precision, suitable for the fine characterization of plant extracts and complex formulations.

To learn more, also discover the dosage of quercetin , the dosage of catechin or the dosage of gallic acid , available in our catalog.

Industrial applications

Phenolic dosage is used in many sectors:

• Food industry : control of the oxidative stability of oils, wines, juices, teas or plant extracts rich in polyphenols.
• Nutraceuticals and food supplements : evaluation of the antioxidant potential of natural ingredients and validation of health claims.
• Animal nutrition : analysis of plant raw materials (plants, fodder, extracts) to characterize their functional value.
• Cosmetics and natural care : determination of phenols responsible for the anti-aging and protective properties of formulas.
• Active materials and packaging : measurement of antioxidants added to limit oxidation and extend product shelf life.

Analytical interest and complementarity

This assay is often combined with other analyses to characterize the complete antioxidant profile of a sample. It can be coupled with:

• an analysis of total antioxidant power (ORAC, DPPH, ABTS) ;
• an analysis of total flavonoids ;
• or even a dosage of condensed tannins for a more specific approach.

The combination of these tests allows a complete evaluation of the bioactive potential of natural extracts and better control of the quality of industrial formulations.

YesWeLab Services

At YesWeLab, we select partner laboratories based on your analytical needs, the matrices to be processed, and the desired methods (spectrophotometry, HPLC, GC-MS, etc.). Our partners are ISO 17025 accredited and/or COFRAC recognized to guarantee the reliability of results.
Thanks to our digital platform , you can centralize your analysis requests, track your samples, and receive your results online.

Since 2020, many manufacturers, distributors and design offices have trusted us for their innovation and quality control projects.

To obtain a quote or additional information on this test, contact our scientific team .