Oxindole alkaloid dosage

The measurement of oxindolic alkaloids allows for the determination of the concentration of these bioactive compounds present in certain medicinal plants, notably Uncaria tomentosa (Cat's Claw). These alkaloids, such as isopteropodine, rhyncophylline, and mitraphylline, are known for their immunomodulatory, anti-inflammatory, and antioxidant properties. Their quantification is essential to guarantee the quality, safety, and standardization of extracts used in nutraceuticals and phytotherapy.

The analysis is performed by high-performance liquid chromatography (HPLC) or by liquid chromatography coupled with mass spectrometry (LC-MS), two reference methods for the separation and identification of alkaloid compounds in complex matrices.

Principle and operation of the method

The sample (dry extract, plant powder, food supplement) is prepared by selective extraction of oxindolic alkaloids using suitable solvents.

• In HPLC-DAD , compounds are separated according to their polarity and detected by UV absorption, allowing for reliable and reproducible quantification.
• In LC-MS , each alkaloid is identified according to its mass/charge ratio (m/z), offering increased specificity, particularly for distinguishing isomers or structurally similar compounds.

These methods guarantee the traceability and conformity of standardized extracts, while ensuring comparability between production batches.

Analytical performance and related analyses

The assay of oxindolic alkaloids is highly sensitive, allowing the detection of low concentrations in a wide range of matrices: dry extracts, plant powders, capsules, and nutraceutical formulations.
HPLC or LC-MS analyses ensure:

• a specific identification of the alkaloids of interest,
• precise quantification for standardization,
• excellent inter-laboratory reproducibility.

For a complete characterization of plant extracts, this analysis can be combined with:

• the dosage of total polyphenols,
• flavonoid dosage
• the dosage of phenolic acids,
• or the dosage of alkylamides , often carried out jointly in studies for the standardization of medicinal plants.

Applications and areas of use

The measurement of oxindolic alkaloids is mainly applicable in the following sectors:

• Nutraceuticals : quality control of Uncaria tomentosa and immunostimulating products.
• Phytotherapy : standardization of plant-based preparations rich in bioactive alkaloids to guarantee efficacy and safety of use.
• Research and development : identification and comparison of the alkaloid profile of different plant species or batches of extracts for the optimization of extraction processes.
• Regulatory control : verification of the conformity of finished products and raw materials according to the standards in force.

These analyses make it possible to certify the scientific and commercial quality of plant-based products, ensuring their consistency and biological efficacy.

The advantages of the YesWeLab service

YesWeLab collaborates with a network of partner laboratories specializing in phytochemical and nutraceutical analyses, the majority of which are ISO 17025 certified and COFRAC accredited. Each project is assigned to the laboratory best suited to the matrix, the method, and the required level of precision.

Thanks to its digital analysis platform, YesWeLab simplifies the management of analytical services: quote requests, sample tracking, and results receipt. Since 2020, numerous manufacturers, laboratories, and distributors have trusted us to master their bioactive compound analyses and enhance the value of their plant extracts.

For any specific request for oxindolic alkaloid dosage, contact our scientific team to obtain tailored support and a personalized quote.