Oleanolic acid dosage

Oleanolic acid assay quantifies this plant-derived pentacyclic triterpene, widely studied for its biological properties. Present in many medicinal plants, it is recognized for its hepatoprotective, anti-inflammatory, antioxidant, and antiviral effects. This analysis is essential for the characterization, standardization, and quality control of natural extracts used in the nutraceutical, cosmetic, and phytopharmaceutical sectors.

Principle and analytical method

Oleanolic acid is typically measured by high-performance liquid chromatography (HPLC) or mass spectrometry (LC-MS) , two techniques that allow for accurate quantification in complex matrices.

• HPLC to their polarity and retention time.
• LC -MS provides fine structural identification and specific detection of characteristic ions of oleanolic acid, even at low concentrations.

These methods can be complemented by phytochemical profile analyses or by coupling with gas chromatography (GC-MS) for other triterpene markers.

Operation and measured parameters

The sample (extract, oil, plant powder, formulation) is subjected to selective organic extraction, followed by chromatographic separation. The UV detector or mass source then identifies the molecule according to its exact mass (m/z 456 for oleanolic acid).
The results are expressed in mg/g or % w/w, depending on the formulation or regulatory compliance needs. The method can be developed and validated according to ISO 17025 requirements or pharmacopoeial standards.

Applications and matrices concerned

The determination of oleanolic acid is carried out in numerous matrices:

• Plant extracts : olive leaves, ginseng roots, hawthorn, chamomile, licorice, etc.
• Vegetable oils and fats : purity and composition control.
• Food supplements : standardization of nutraceutical active ingredients.
• Creams, balms and cosmetic treatments : evaluation of antioxidant efficacy and stabilization of the formulation.

This analysis is frequently integrated into studies of triterpene dosage or analysis of complex plant extracts , particularly alongside ursolic acid , a structurally similar molecule.

Interest for industrialists

The analysis of oleanolic acid allows:

• ensure the standardization of plant extracts ,
• ensure traceability and regulatory compliance (ISO, pharmacopoeias, biological specifications),
• assess the content of active ingredients in nutraceutical or cosmetic formulations,
• validate the stability and purity of natural raw materials.

This service is particularly suitable for manufacturers of food supplements, natural health products, plant extracts or dermocosmetic treatments.

YesWeLab Expertise

At YesWeLab , we collaborate with a rigorously selected network of partner laboratories, certified and/or accredited ISO 17025 and COFRAC , specialized in the analysis of natural compounds.
Our scientific team selects the method best suited to your samples and your objectives (quality control, validation, regulatory compliance).
Since 2020, many players in nutraceuticals , phytotherapy and natural cosmetics have trusted us for the management and monitoring of their analyses via our digital platform.

Contact our team for a personalized quote or to discuss a specific analytical need.