Oleanolic acid assay

The measurement of oleanolic acid allows for the quantification of this pentacyclic triterpene of plant origin, which has been extensively studied for its biological properties. Present in numerous medicinal plants, it is recognized for its hepatoprotective, anti-inflammatory, antioxidant, and antiviral effects. This analysis is essential for the characterization, standardization, and quality control of natural extracts used in the nutraceutical, cosmetic, and phytopharmaceutical sectors.

Principle and analytical method

Oleanolic acid is generally measured by high-performance liquid chromatography (HPLC) or by mass spectrometry (LC-MS) , two techniques allowing precise quantification in complex matrices.

• HPLC to their polarity and retention time.
• LC -MS provides fine structural identification and specific detection of ions characteristic of oleanolic acid, even at low concentrations.

These methods can be complemented by phytochemical profiling analyses or by coupling with gas chromatography (GC-MS) for other triterpene markers.

Functioning and measured parameters

The sample (extract, oil, vegetable powder, formulation) undergoes selective organic extraction, followed by chromatographic separation. The UV detector or mass spectrometer then identifies the molecule according to its exact mass (m/z 456 for oleanolic acid).
The results are expressed in mg/g or w/w %, depending on the formulation requirements or regulatory compliance. The method can be developed and validated according to ISO 17025 requirements or pharmacopoeia standards.

Applications and matrices concerned

The determination of oleanolic acid is carried out in numerous matrices:

• Plant extracts : olive leaves, ginseng roots, hawthorn, chamomile, licorice, etc.
• Vegetable oils and fats : purity and composition control.
• Dietary supplements : standardization of nutraceutical active ingredients.
• Creams, balms and cosmetic treatments : evaluation of antioxidant efficacy and formulation stabilization.

This analysis is frequently integrated into studies of triterpene quantification or analysis of complex plant extracts , particularly alongside ursolic acid , a structurally similar molecule.

Interest for manufacturers

Analysis of oleanolic acid allows us to:

• to guarantee the standardization of plant extracts ,
• ensure traceability and regulatory compliance (ISO, pharmacopoeias, biological specifications),
• assess the content of active ingredients in nutraceutical or cosmetic formulations,
• validate the stability and purity of natural raw materials.

This service is particularly suited to manufacturers of food supplements, natural health products, plant extracts or dermo-cosmetic care.

YesWeLab Expertise

At YesWeLab , we collaborate with a carefully selected network of partner laboratories, certified and/or accredited to ISO 17025 and COFRAC , specializing in the analysis of natural compounds.
Our scientific team selects the method best suited to your samples and objectives (quality control, validation, regulatory compliance).
Since 2020, numerous players in the nutraceutical , phytotherapy , and natural cosmetics have entrusted us with the management and monitoring of their analyses via our digital platform.

Contact our team to obtain a personalized quote or to discuss a specific analytical need.