Skin corrosion – OECD 431

Skin corrosion analysis assesses the corrosive potential of a product or substance on human skin. It is based on an internationally validated in vitro model using reconstituted human epidermal tissue. This method, governed by OECD Guideline 431, is a reliable alternative to animal testing to meet regulatory requirements in cosmetics, chemistry, and detergents.

Purpose of the test

This test is used to determine whether a product causes irreversible damage to the skin, characterized by the destruction of the layers of the epidermis. It is used to:

• Classify corrosive substances according to the GHS or CLP classification systems
• Ensure the safety of formulations before placing them on the market
• Comply with regulatory requirements (REACH, cosmetic regulations, etc.)
• Avoid in vivo testing by demonstrating the absence of skin hazard

OECD 431 Methodology

The skin corrosion test is performed on reconstructed human skin (RhE) models. The principle consists of applying the tested substance to the tissues for a predefined period (3 min, then 60 min). Cell viability is then measured spectrophotometrically using a metabolic indicator (e.g., MTT).

The results allow the substance to be classified as:

• Corrosive (Category 1A, 1B, 1C)
• Non-corrosive (no tissue destruction)

Affected matrices

This test is applicable to a wide variety of products:

• Cosmetics : exfoliants, peels, extreme pH creams
• Maintenance and industrial products : detergents, solvents, strippers
• Raw chemicals : acids, bases, concentrated formulations

Related techniques

• Culture of reconstituted human epithelial tissues
• Spectrophotometric reading of viability (MTT)
• Compliance with OECD 431 standardized conditions

Why choose YesWeLab?

At YesWeLab, we connect you with laboratories that are experts in in vitro toxicology and have a perfect command of OECD protocols, including guidelines 431 (corrosion) and 439 (irritation). Our partners have validated RhE models , calibrated spectrophotometry equipment, and are often ISO 17025 certified .

We support you in selecting the right regulatory test, planning your trials, and managing usable reports for your REACH dossiers, cosmetic dossiers or export requests.

See also:

• Skin irritation test – OECD 439
• Eye irritation test – OECD 437, 438, 492
• Challenge test

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