Skin corrosion – OECD 431

Skin corrosion analysis assesses the corrosive potential of a product or substance on human skin. It relies on an internationally validated in vitro model using reconstituted human epidermal tissue. This method, governed by OECD guideline 431, provides a reliable alternative to animal testing for meeting regulatory requirements in the cosmetics, chemical, and detergent industries.

Test objective

This test determines whether a product causes irreversible skin damage, characterized by the destruction of the epidermal layers. It is used to:

• Classify corrosive substances according to the GHS or CLP classification systems
• Ensuring the safety of formulations before they are placed on the market
• Comply with regulatory requirements (REACH, cosmetics regulations, etc.)
• Avoid in vivo testing by demonstrating the absence of skin risk

OECD Methodology 431

The skin corrosion test is performed on reconstructed human skin (RhE) models. The principle involves applying the substance being tested to the tissues for a predetermined duration (3 min, then 60 min). Cell viability is then measured by spectrophotometry using a metabolic indicator (e.g., MTT).

The results allow us to classify the substance as:

• Corrosive (Category 1A, 1B, 1C)
• Non-corrosive (does not cause tissue damage)

Affected matrices

This test is applicable to a wide variety of products:

• Cosmetics : exfoliants, peels, extreme pH creams
• Cleaning and industrial products : detergents, solvents, paint strippers
• Raw chemical substances : acids, bases, concentrated formulations

Related techniques

• Culture of reconstituted human epithelial tissues
• Spectrophotometric reading of viability (MTT)
• Compliance with OECD Standardized Conditions 431

Why choose YesWeLab?

At YesWeLab, we connect you with expert in vitro toxicology laboratories that have a thorough understanding of OECD protocols, particularly guidelines 431 (corrosion) and 439 (irritation). Our partners have validated RhE models , calibrated spectrophotometry equipment, and are often ISO 17025 certified .

We support you in selecting the right regulatory test, planning your tests, and managing usable reports for your REACH dossiers, cosmetic dossiers or export applications.

See also:

• Skin irritation test – OECD 439
• Eye irritation test – OECD 437, 438, 492
• Challenge test

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