Nobiletin (flavone) dosage

Nobiletin flavone naturally present in citrus peels, particularly mandarin (Citrus reticulata). It is recognized for its anti-inflammatory, hypolipidemic, neuroprotective and anticancer effects.

Measuring nobiletin levels is essential for:

• Standardize plant extracts used in nutraceuticals or food supplements
• Control the content of active ingredients in enriched formulations
• Valuing citrus by-products within a circular economy framework
• Meeting the requirements of regulatory or labeling documentation
• Verify the conformity of extracts for therapeutic or functional use

Methodology

The analysis is performed by HPLC-DAD (UV detection around 330 nm) or LC-MS/MS for improved sensitivity and specificity. The protocol includes:

• Hydroalcoholic or methanolic extraction from solid or liquid matrices
• Filtration and possible purification (SPE or centrifugation)
• Chromatographic analysis with calibration curve on nobiletin standard

The choice of method depends on the complexity of the matrix and the desired level of precision (quantification or simple detection).

Affected matrices

• Citrus peels (mandarin, sweet orange, kumquat)
• Plant extracts, standardized or unstandardized
• Dried or freeze-dried plant powders
• Nutraceutical capsules, tablets or liquids
• Functional drinks enriched with flavonoids
• Natural cosmetic formulations containing citrus extracts

Industrial applications

• Dietary supplements and antioxidant extracts
• Functional nutrition (drinks, fortified powders)
• Natural cosmetics (anti-aging, radiance, antioxidant skincare)
• Research in nutrition, health, and plant pharmacology
• Valorization of co-products from the juice and essential oil industry

Why choose YesWeLab?

YesWeLab connects you with laboratories specializing in the analysis of citrus flavonoids , accredited to ISO 17025. Our partners have the necessary equipment (HPLC, LC-MS/MS) and advanced expertise in developing analytical methods for complex extracts.

See also:

• Hesperidin Dosage
• Narirutin dosage
• Diosmin dosage