Neohesperidin dosage

Neohesperidin is a flavanone naturally present in bitter citrus fruits, particularly grapefruit , bitter orange , and lime . It is often transformed into neohesperidin dihydrochalcone (NHDC) , an intense sweetener authorized for human consumption.

Neohesperidin levels can be measured to:

• Controlling the quality of citrus extracts (flavonoid standardization)
• Verify the actual concentration of NHDC in sugar formulations
• Meeting the requirements of food supplements, functional drinks or low-fat products
• Evaluate the purity of flavanone extracts or isolates

Analytical Methodology

The analysis is carried out by HPLC-DAD (UV detection in wavelengths from 280 to 330 nm), according to a protocol validated for food and plant matrices.

• Hydroalcoholic or methanolic extraction
• Possible purification by filtration or solid phase (SPE)
• Separation on C18 column in reverse phase
• Quantification using a pure neohesperidin standard

If necessary, the analysis can be coupled with LC-MS/MS identification (especially for complex extracts or very sweet matrices).

Frequently analyzed matrices

• Dried or concentrated citrus powders
• Standardized or raw plant extracts (citrus, grapefruit, etc.)
• Flavonoid-based or NHDC-based food supplements
• Natural sweeteners intended for human consumption
• Fortified functional beverages
• Cosmetic products containing citrus extracts

Industrial applications

• Standardization of plant extracts for the nutraceutical market
• Development of natural sweet formulations
• Verification of NHDC content for additive regulations (E959)
• Research on the antioxidant and hepatoprotective properties of flavanones
• Quality analysis in clean label products

Why use YesWeLab?

YesWeLab connects you with the best laboratories specializing in flavonoid analysis, equipped with HPLC-DAD, HPLC-MS/MS and ISO 17025 accredited for physicochemical analyses on complex matrices.

See also:

• Naringin dosage