Neohesperidin dosage

Neohesperidin is a flavanone naturally present in bitter citrus fruits, including grapefruit , bitter orange , and lime . It is often converted into neohesperidin dihydrochalcone (NHDC) , an intense sweetener approved for human nutrition.

The dosage of neohesperidin allows:

• Control the quality of citrus extracts (flavonoid standardization)
• Check the actual NHDC concentration in sweet formulations
• Meet the requirements of food supplements, functional drinks or light products
• Assess the purity of flavanone extracts or isolates

Analytical methodology

The analysis is carried out by HPLC-DAD (UV detection in wavelengths from 280 to 330 nm), according to a protocol validated for food and plant matrices.

• Hydroalcoholic or methanolic extraction
• Possible purification by filtration or solid phase (SPE)
• Separation on C18 column in reverse phase
• Quantification using a pure neohesperidin standard

If necessary, the analysis can be coupled with LC-MS/MS identification (particularly for complex extracts or very sweet matrices).

Frequently analyzed matrices

• Dried or concentrated citrus powders
• Titrated or raw plant extracts (citrus, grapefruit, etc.)
• Flavonoid-based food supplements or NHDC
• Natural sweeteners for human consumption
• Fortified functional drinks
• Cosmetic products containing citrus extracts

Industrial applications

• Standardization of plant extracts for the nutraceutical market
• Development of natural sweet formulations
• Verification of NHDC content for additive regulation (E959)
• Research on the antioxidant and hepatoprotective properties of flavanones
• Quality analysis in clean label products

Why use YesWeLab?

YesWeLab connects you to the best laboratories specialized in flavonoid analysis, equipped with HPLC-DAD, HPLC-MS/MS and ISO 17025 accredited for physicochemical analyses on complex matrices.

See also:

• Naringin Dosage