Melilotic acid dosage

Melilotic acid analysis is performed primarily by high-performance liquid chromatography (HPLC-DAD). This method allows for fine separation of coumarin compounds present in plant extracts and precise quantification, even at trace levels.

In some cases, particularly for complex matrices or multi-component extracts, the LC-MS (liquid chromatography coupled with mass spectrometry) method can be used to enhance the specificity and sensitivity of the assay.

Analyzed matrices

The dosage of melilotic acid applies to several types of matrices:

• Dry or fluid extracts of sweet clover
• Medicinal plant powders
• Infusions and herbal teas for circulatory purposes
• Venous-active food supplements
• Combined natural formulations (red vine, witch hazel, horse chestnut, etc.)

This dosage is often included in a quality specification or a regulatory control plan linked to the safety of food supplements.

Importance of dosage in circulatory formulations

Melilotic acid is one of the natural compounds used to prevent venous circulation disorders, particularly in cases of heavy legs, varicose veins, or chronic venous insufficiency. The dosage of this active ingredient guarantees a consistent and controlled concentration in standardized extracts, allowing for an effective formulation that meets both user expectations and regulatory requirements.

Additional analyses available at YesWeLab

YesWeLab also offers the dosage of several other coumarin and flavonoid molecules frequently associated with melilotic acid in herbal medicine products:

• Coumarin
  → Naturally present in sweet clover, cinnamon or tonka bean, it is often measured to assess potential toxicity or the total concentration of coumarin compounds.
• Fraxine
  → Furanocoumarin from ash (Fraxinus excelsior), used for its antioxidant and circulatory properties.
• Xanthotoxin
  → Photosensitizing molecule from bergamot, celery or parsley, to be monitored for extracts for cosmetic or food use.
• Caffeic acid
  → Antioxidant polyphenol often associated with venous extracts to reinforce the protective effect on capillaries.

These analyses can be combined as part of a polyphenolic profile or multi-active screening, particularly for formulations based on several plants.

YesWeLab, your analytical partner

At YesWeLab, we collaborate with a carefully selected network of specialized, certified and/or accredited laboratories (ISO 17025, COFRAC, GMP). Our services are tailored to the needs of professionals in the fields of phytotherapy, nutraceuticals, cosmetics, and natural health.

Thanks to our digital platform, you can easily submit your samples, track your requests, and receive your results quickly. Since 2020, we have supported numerous manufacturers, brands, and design offices in their analytical projects.

Start your analysis today

Are you working on a sweet clover-based formulation? Would you like to validate the quality of a vasoprotective plant extract? Request a personalized quote now via our platform or contact our scientific team for tailored support.