Luteolin assay

Luteolin is a natural flavone with remarkable biological properties, found in many medicinal and aromatic plants such as thyme, artichoke, celery, rosemary, and chamomile. It is recognized for its antioxidant, anti-inflammatory, antiviral, and neuroprotective effects, making it a marker of major interest for the formulation of dietary supplements, standardized extracts, and herbal products.

Luteolin measurement is essential to guarantee the quality of plant extracts, to verify the content of active ingredients, or to carry out stability and regulatory compliance studies in the nutraceutical, cosmetic or phytotherapeutic fields.

Analytical method: HPLC-DAD or LC-MS/MS

Luteolin analysis is performed by HPLC (high-performance liquid chromatography) with diode array detection (DAD) or by LC-MS/MS (liquid chromatography coupled to tandem mass spectrometry), depending on the level of sensitivity or the complexity of the matrices.

The steps include:

• Extraction of phenolic compounds from dry or liquid matrices
• Optimized chromatographic separation
• A specific and sensitive detection method, allowing for the quantification of free luteolin even at low concentrations

All methods are deployed in partner laboratories accredited to ISO 17025 and compliant with COFRAC standards, ensuring the reliability and traceability of results.

Analyzed matrices

Luteolin quantification applies to a wide range of plant matrices and formulated products:

• Extracts of medicinal plants (artichoke, thyme, rosemary, chamomile, etc.)
• Plant powders and raw materials
• Flavonoid-based food supplements
• Infusions, herbal teas, decoctions
• Cosmetic products containing antioxidant extracts

This analysis is essential in the development of products with anti-aging, anti-inflammatory or nervous system protective effects.

Industrial applications and purposes

Measuring luteolin levels is particularly useful for:

• Standardize plant extracts according to defined quality criteria (mg/g or mg/mL)
• Monitor regulatory compliance (particularly with regard to health claims)
• Optimizing cosmetic formulations or antioxidant supplements
• Conduct comparative studies on flavone content between different botanical sources
• Evaluate the impact of extraction or drying processes on the active ingredient content

It is also a traceability criterion to differentiate between plant origins or manufacturing batches.

Additional analyses available at YesWeLab

To further evaluate flavones and polyphenols, we propose:

• Assay of luteolin-7-O-glucoside – its glycosylated form commonly found in chamomile and artichoke
• Luteolin-7-glucuronide assay – a metabolite useful in pharmacokinetics and bioavailability
• Apigenin dosage – a flavone similar to luteolin, with anti-inflammatory properties
• Total flavonoid assay – global method for complex extracts

Why choose YesWeLab for luteolin testing?

At YesWeLab , we facilitate access to high-quality analyses through a network of over 200 partner laboratories, rigorously selected for their analytical expertise. We offer:

• Personalized scientific support
• Controlled deadlines, tailored to your development or quality control needs
• A centralized, intuitive and secure digital platform for managing analyses
• Compliance with the most demanding analytical standards (ISO 17025, COFRAC)

Do you need a luteolin dosage in your extracts?

Contact our team for a personalized quote or to discuss your analysis project. Our experts will help you define the method best suited to your matrix and objectives.