Lupeol Dosage

Lupeol measurement quantifies this naturally occurring pentacyclic triterpene, present in many medicinal plants and vegetable oils such as neem ( Azadirachta indica ), aloe ( Aloe vera ), olive, grape and birch. Used for its anti-inflammatory, antioxidant and healing properties, lupeol is a marker of quality and biological activity in plant extracts and cosmetic or nutraceutical formulations.

This analysis is carried out by HPLC or LC-MS/MS , reference methods for the precise quantification of triterpenes in complex matrices of natural origin.

Lupeol: a bioactive triterpene of industrial interest

Lupeol is a molecule from the triterpene family, derived from the lupane skeleton. It is found in many plant species, including resins, oils, bark, and leaves. It plays a key biological role in protecting plants from environmental stress and has recognized therapeutic effects.

Biochemically, lupeol exhibits a broad spectrum of activities:

• anti-inflammatory effect by inhibition of inflammatory mediators,
• antioxidant and protective activity of cell membranes,
• healing and skin repairing properties,
• antiproliferative and immunomodulatory effect,
• mild antimicrobial action depending on matrix and concentration.

Due to these multiple effects, lupeol has become a reference marker in the analyses of medicinal plant extracts, vegetable oils and formulations for dermatological or nutraceutical purposes.

Objectives and interest of lupeol dosage

The analysis of lupeol aims to:

• determine the concentration of active triterpenes in plant extracts or ingredients,
• check the conformity of batches according to established quality standards,
• control the purity and stability of the compound during processing,
• validate functional claims related to anti-inflammatory or restorative properties,
• standardize finished products to ensure consistent effectiveness.

The dosage is applicable to extracts of aloe, neem, olive, birch, as well as cosmetic or nutraceutical formulations containing triterpene fractions.

Analytical methods used

The quantification of lupeol is based on chromatographic techniques adapted to apolar and low volatility compounds:

• HPLC (high-performance liquid chromatography) : Used to separate and quantify triterpenes based on their UV absorption spectrum. This method is particularly suitable for oily matrices or crude extracts.
• LC-MS/MS (liquid chromatography coupled with tandem mass spectrometry) : reference method for the identification and precise quantification of lupeol, offering optimal sensitivity and high specificity.

These methods can be coupled with a sample preparation step (liquid-liquid extraction or SPE) in order to concentrate the analytes and eliminate interferences.

For a complete characterization of the extracts, YesWeLab also offers the dosage of total triterpenes, the dosage of betulinol, or the dosage of ursolic acid , allowing to obtain a global vision of the triterpene profile of the samples.

Industrial and scientific applications

Lupeol dosage is used in several areas:

• Nutraceutical : control of the lupeol content in plant extracts used for the formulation of food supplements for anti-inflammatory or antioxidant purposes.
• Natural cosmetics : evaluation of the quality of active ingredients in restorative, anti-aging, soothing or purifying treatments based on vegetable oils or plant extracts rich in triterpenes.
• Phytochemical research : study of the triterpene profile of new plant species and analysis of the stability of lupeol in complex formulations.
• Pharmaceutical and dermocosmetic industry : dosage validation in the quality control and product development phases.

Lupeol is often analyzed together with other bioactive compounds such as β-sitosterol, oleanolic acid or total saponins , for an integrated approach to the chemical composition of extracts.

Carrying out analyses at YesWeLab

YesWeLab entrusts the determination of lupeol to partner laboratories specialized in chromatographic analysis and the characterization of natural compounds. These laboratories have state-of-the-art HPLC and LC-MS/MS equipment, guaranteeing reliable and reproducible results.

Most YesWeLab partners are certified or accredited according to the ISO 17025 standard and the COFRAC standard, ensuring compliance and traceability of analyses.

Our scientific team supports manufacturers and formulators at every stage: choosing the method, optimizing the analytical protocol, interpreting results, and monitoring ingredient stability. Thanks to the YesWeLab digital platform, analysis management is simplified: online quotes, sample tracking, and quick access to analytical reports.

Since 2020, many players in natural cosmetics, nutraceuticals and phytochemistry research have trusted YesWeLab for the characterization and validation of their extracts rich in triterpenes and bioactive plant compounds.

Technical data

• CAS No.: 545-47-1
• Methods: HPLC, LC-MS/MS
• Expertise: Physicochemical, nutritional
• Sectors of activity: Nutraceuticals, plants, natural cosmetics
• Measured parameters: Lupeol and associated triterpenes

For any specific request, contact our team to obtain a personalized quote and analytical recommendations tailored to your plant extracts or natural formulations.