Ophiopogonin D assay

Ophiopogonin D is a major saponoside isolated from the plant Ophiopogon japonicus , traditionally used in Asian pharmacopoeia.

Known for its protective effects on the respiratory tract and its antioxidant properties, this molecule is attracting increasing interest in the fields of nutraceuticals, phytotherapy and the formulation of food supplements.

The measurement of ophiopogonin D is essential to guarantee the standardization of plant extracts , validate the quality of raw materials, and ensure the consistency of finished formulations. It thus contributes to strengthening the reliability and conformity of products intended for international markets.

Analytical methods

Ophiopogonin D analysis is performed using high-performance liquid chromatography techniques:

• HPLC-DAD for UV separation and detection of saponosides, suitable for routine controls.
• LC-MS or LC-MS/MS , which offer increased specificity and sensitivity, allowing precise quantification even in complex matrices.

These state-of-the-art methods guarantee reproducible results that comply with regulatory and industrial requirements.

Affected matrices

Ophiopogonin D can be measured in different matrices:

• Standardized plant extracts from Ophiopogon japonicus
• Dried plant powders
• Dietary supplements in capsules, tablets or liquid solutions
• Complex nutraceutical formulations incorporating several plant-based active ingredients

Industrial applications

• Nutraceuticals: formulation and standardization of supplements intended for respiratory well-being.
• Phytotherapy: promoting traditional preparations based on Ophiopogon.
• Quality control: batch tracking, raw material validation and finished product conformity.
• Research and development: study of the stability and biological activity of saponosides.

Additional analyses

Ophiopogonin D levels can be combined with other analyses of saponins and bioactive compounds for a comprehensive assessment:

• Diosgenin assay
• Glycyrrhizin assay
• Total polyphenol content

This broader approach allows for a better characterization of the synergy between the different plant actives and strengthens the scientific value of the extracts.

Regulations and quality issues

The establishment of reliable and standardized analyses for ophiopogonin D is essential in a demanding regulatory environment. Standardizing plant extracts is a crucial step to ensure their compliance with the quality, traceability, and safety standards expected in the nutraceutical and pharmaceutical sectors.

YesWeLab expertise

At YesWeLab, we work with a network of specialized partner laboratories, ISO 17025 certified and COFRAC accredited, selected based on the matrix and analytical methods required. Our digital platform simplifies the management of your analyses: sample submission, service tracking, and centralized results reception.

Our scientific team is available to support you in your projects, define appropriate protocols and meet your specific requirements in terms of sensitivity, speed and regulatory compliance.