Isorhamnetin dosage

Isorhamnetin is a flavonol naturally present in many medicinal plants and plant-based foods. A methylated derivative of quercetin , this compound belongs to the flavonoid , known for their antioxidant, anti-inflammatory, and vasoprotective properties .
Isorhamnetin levels are essential for assessing the quality and standardization of plant extracts used in dietary supplements, nutraceutical products, and herbal formulations.

Principle and operation of the method

The analysis of isorhamnetin relies on high-precision chromatographic techniques, such as high-performance liquid chromatography (HPLC) or liquid chromatography coupled with mass spectrometry (LC-MS) .

The analysis protocol typically includes:

• selective extraction of flavonoids from the plant matrix (maceration, sonication or solvent-assisted extraction),
• Chromatographic separation according to the polarity of the compounds,
• and detection by diode array (HPLC-DAD) or by mass spectrometry (LC-MS/MS) , offering reliable quantification even at trace levels.

The HPLC-DAD method is preferred for routine assay and standardization of extracts , while LC-MS is used for the specific identification of co-extracted flavonoids or related metabolites (quercetin, kaempferol, rhamnetin…).

Analytical performance and related analyses

Quantifying isorhamnetin allows for the characterization of the phenolic compound content of extracts and guarantees their authenticity.
For a complete flavonoid profile analysis, this service can be combined with other tests from the YesWeLab catalog, such as:

• the dosage of quercetin ,
• the dosage of rutin ,
• the dosage of kaempferol,
• or the dosage of total flavonoids ,
  in order to provide a complete chemical signature of the plant extract .

These combined analyses are essential for phytotherapeutic research , the formulation of supplements or the quality control of raw materials .

Applications and areas of use

Isorhamnetin testing is applicable to various sectors:

• Nutraceuticals : standardization of flavonoid-rich plant extracts for the formulation of antioxidant supplements.
• Phytotherapy : verification of the isorhamnetin content in tinctures and extracts used for their vasculoprotective and anti-inflammatory effects.
• Research and development : study of the bioavailability and metabolism of flavonoids in biological models.

The most common matrices include plant extracts , dry powders , tinctures , capsules , and liquid formulations .

The advantages of the YesWeLab service

YesWeLab collaborates with a network of ISO 17025 and COFRAC accredited laboratories specializing in chromatography and mass spectrometry applied to phytochemicals.
Each request is directed to the most suitable laboratory based on the matrix, the desired method, and the sensitivity requirements.

Thanks to its digital platform , YesWeLab simplifies analysis management: sample submission, real-time tracking, and rapid results delivery.
Since 2020, numerous manufacturers and distributors have trusted YesWeLab for their quality control and standardization studies of natural extracts .

For any specific needs regarding isorhamnetin assay, contact our scientific team for a personalized quote and support tailored to your analytical objectives.