Isogentisine assay

Isogentisin is a xanthone characteristic of gentian roots (Gentiana spp.), a plant known for its bitter properties and long used in herbal medicine and the food industry. This compound is particularly studied as a marker molecule in the standardization of extracts of gentian and other bitter plants. Its measurement helps guarantee the quality of plant raw materials and ensures consistency in the formulation of dietary supplements, bitter beverages, pharmaceutical preparations, and tinctures.

Importance of Isogentisin Assay:
Isogentisin analysis is of particular importance in several fields:
The nutraceutical and dietary supplement sector, where it is used to standardize gentian extracts used for their digestive and stimulant effects.
The beverage industry, for quality control of herbal bitters, aperitifs, and tonics.
Phytotherapy and herbalism, for the identification and quantification of the compound in therapeutic preparations.
Compliance control, for verifying the stability of finished products and monitoring manufacturing processes.

Analytical Methods:
Isogentisin is determined by high-performance liquid chromatography (HPLC-DAD) or LC-MS, two reference techniques for the analysis of polyphenolic and xanthone compounds. HPLC-DAD allows for the separation and quantification of isogentisin by detecting its characteristic UV spectrum. LC-MS offers increased sensitivity and enables reliable quantification, even in complex matrices such as hydroalcoholic extracts, tinctures, or concentrated formulations. These methods guarantee high precision and reproducibility, essential for quality control and research.

Matrices concerned
Isogentisin dosage is particularly suitable for extracts of gentian and other bitter plants, liquid preparations such as tinctures or hydroalcoholic extracts, finished products such as bitter drinks, liqueurs and food supplements, as well as root powders and dry formulations.

Related analyses in our catalog:
Isogentisin analysis can be complemented by other relevant analyses such as gentiopicroside analysis , another major marker of gentian; swertiamarin analysis , a bitter iridoid also used as a quality indicator; luteolin analysis , a flavonoid present in many medicinal plants; or even total flavonoid analysis for a comprehensive characterization of the extracts. This combined approach allows for complete standardization of plant extracts and better control of raw material quality.

YesWeLab Support
At YesWeLab, we work with a network of specialized, certified, and/or accredited partner laboratories (ISO 17025, COFRAC, etc.). We select the most suitable laboratory based on your analytical needs, the type of matrix, and the required methods. Our scientific team supports you at every stage, from defining your analytical needs to interpreting the results. Since 2020, we have earned the trust of numerous manufacturers, distributors, and engineering firms thanks to our digital platform, which centralizes the management of your analyses and simplifies sample tracking.

Isogentisin analysis is an essential tool for manufacturers in the nutraceutical, phytotherapy, and food industries. It not only ensures product compliance with quality standards but also guarantees the consistency and traceability crucial in a market where transparency and reliability are paramount. Contact our team today to schedule your analyses and safeguard the quality of your plant extracts and formulations.