Lipocalin-2 assay

Lipocalin -2 (or NGAL – Neutrophil Gelatinase-Associated Lipocalin) is a protein of the lipocalin family involved in the regulation of innate immunity and inflammatory responses. It is notably expressed by neutrophils and certain epithelial cells in response to stress, infection or acute inflammation.

Lipocalin-2 assay is used as an immune biomarker in the assessment of animal health status, monitoring of immune performance or development of functional formulations for animal nutrition.

Dosage objectives

• Monitor inflammatory or infectious status in animals
• Evaluate the effect of immunostimulating ingredients (nutritional supplements)
• Validating biomarkers in veterinary clinical trials
• Characterize nutritional or physiological matrices (serum, milk, plasma)

Lipocalin -2 is also being studied in the context of oxidative stress, the gut-immune axis, or metabolic disorders, including in livestock and laboratory animals.

Analytical methods

The dosage is carried out mainly by:

• ELISA (Enzyme-Linked Immunosorbent Assay): the standard immuno-enzymatic method for the quantification of specific proteins in biological samples. It allows sensitive and reproducible detection.
• Spectrophotometry or other immunochemical techniques (immunoturbidimetry, immunoprecipitation), depending on the type of matrix and the expected concentration.

The ELISA kits used are validated for specific animal species (pig, ruminant, poultry, etc.) or custom-developed for R&D applications.

Affected matrices

• Animal serums and plasmas
• Protein extracts or functional products (nutritional supplements)
• Experimental samples (milk, feces, tissues)

This assay can be coupled with other biomarkers such as haptoglobin , CRP or inflammatory cytokines . It is integrated into complete panels for monitoring immunity in animals.

Industrial applications

• Formulation and validation of nutraceutical products for animals
• Preclinical/clinical studies on the efficacy of veterinary food supplements
• Animal health programs and infectious disease prevention
• Monitoring the metabolic and immune performance of livestock

Also see our sheet on the dosage of erythrocyte glutathione peroxidase (GSH-PxE) , another marker of oxidative stress in animal health.

Why use YesWeLab?

YesWeLab selects for you the best laboratories specialized in veterinary immunological analyses, ISO 17025 accredited and equipped with reagents adapted to the species studied. Our team supports you:

• In choosing the method suited to your objective
• For the logistical organization and quality monitoring of your analyses
• With personalized and responsive scientific support

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