Lipase assay

Lipases (EC 3.1.1.3) are hydrolase enzymes involved in the breakdown of lipids into fatty acids and glycerol. They play a key role in biological, fermentation, and industrial processes technical products, and the enzymatic valorization of natural extracts.

Lipase assays allow measurement of their specific enzymatic activity , which is essential for quality control of food products, enzyme extracts, biotechnological formulations or nutraceutical supplements.

Analytical methods: spectrophotometry, HPLC and ELISA

The choice of method depends on the type of sample and the level of precision required:

• Spectrophotometry : a classic method for measuring enzymatic activity based on the hydrolysis of a substrate (e.g., p-nitrophenyl palmitate) and the colorimetric detection of the products formed.
• HPLC : reference technique for quantifying hydrolysis products or kinetic monitoring of lipase activity in complex mixtures.
• ELISA : suitable for the specific immunological detection of lipases in biological matrices or commercial formulations.

These approaches allow for obtaining reliable quantitative data on lipase activity or concentration, according to current analytical standards.

Applications and areas concerned

Lipase assays are used in many sectors:

• Agri-food : control of enzymatic activity in fermented products (cheeses, yogurts, oils).
• Nutraceuticals and food supplements : verification of the digestive enzyme content in formulations.
• Animal nutrition : evaluation of enzymes used to improve fat digestibility.
• Biotechnology and enzyme industry : monitoring of production or purification of technical enzymes.
• Plants and natural extracts : study of lipase activity in complex plant or enzymatic extracts.

This service can be combined with amylase or protease assays for a complete enzymatic characterization .

Analyzed matrices

YesWeLab's partner laboratories perform lipase assays on:

• Plant and animal extracts
• Enzyme powders and formulations
• Fermented products and food supplements
• Technical or industrial solutions

Each method is adapted according to the matrix , the type of enzyme and the expected units of enzymatic activity (IU/mg, IU/g, etc.).

YesWeLab expertise

At YesWeLab , we collaborate with a network of ISO 17025 and COFRAC accredited laboratories , specializing in enzymatic and biochemical analyses .

Our scientific team supports you in:

• Choosing the analytical method best suited to your matrix
• preparation and packaging
• Online tracking of your analyses via our digital platform

Since 2020, YesWeLab has been supporting food, nutrition and biotechnology companies in quality control and validation of their enzymatic products .

Order a lipase analysis

Do you want to evaluate the activity or concentration of lipases in your products?
Submit your request via our platform or contact our scientific team for personalized support .

→ Add to quote now to get a quick and accurate quote.