Lignin determination

Lignin is a complex phenolic polymer naturally present in the cell wall of plants. It plays a fundamental role in the rigidity, mechanical strength, and protection of plant tissues. Its measurement is essential for assessing the indigestible fiber content in plant raw materials, particularly in the context of animal nutrition, biomass recovery, or the quality control of botanical extracts.

Objectives of the analysis

Lignin dosage allows:

• Characterize the digestibility of fibers in forages and plant co-products
• Determine the proportion of non-assimilable plant matter in animal feed
• Assess the quality of biomass for the production of bioenergy or bio-sourced materials
• Standardize plant extracts in phytotherapy or nutraceuticals

This parameter is essential for animal feed manufacturers, natural supplement manufacturers and biomaterial producers.

Available analytical methods

Van Soest Method (ADL – Acid Detergent Lignin)

The ADL method is the most commonly used in animal nutrition. It is based on a sequential extraction of the different fibrous fractions: NDF (Neutral Detergent Fiber), ADF (Acid Detergent Fiber), then lignin. It allows a standardized evaluation of residual lignin.

Gravimetry after acid hydrolysis

This method involves isolating crude lignin by acid hydrolysis, followed by gravimetric determination. It is recommended for processed matrices rich in cellulose, or complex extracts.

HPLC

High-performance liquid chromatography (HPLC) analysis is used in some cases to quantify specific lignin subunits or monitor structural changes in purified extracts.

Affected matrices

Lignin determination applies to many matrices:

• Raw fodder (silage, alfalfa, corn, hay)
• Plant-based raw materials for animal feed
• Plant extracts (tinctures, powders, supplements)
• Lignocellulosic biomass (chips, plant waste, agricultural residues)
• Finished products containing complex fibers

Industrial interest

In animal feed, lignin content is a limiting factor in digestibility. Accurate measurement allows for the formulation of fiber-balanced rations. In bioprocesses, it can be used to predict the yield of energy or by-products from biomass.

Lignin analysis is also often combined with additional assays such as cellulose, NDF/ADF fibers, or crude ash. See, for example, the sheet on Determination of Total, Insoluble, and Soluble Dietary Fiber.

Why go through YesWeLab?

YesWeLab provides you with a network of laboratories specializing in nutritional and plant analyses. Most of our partners are ISO 17025 certified and use methods validated according to AFNOR or Van Soest standards. We support you every step of the way:

• Selecting the right method for your sample
• Logistics monitoring and traceability via our digital platform
• Fast return of results

Request your quote

Would you like to measure lignin in your samples or integrate this analysis into a comprehensive fiber assessment? Contact our team via the YesWeLab platform. We will direct you to the laboratory best suited to your requirements and deadlines.