Lignin assay

Lignin is a complex phenolic polymer naturally present in plant cell walls. It plays a fundamental role in the rigidity, mechanical strength, and protection of plant tissues. Its measurement is essential for assessing the indigestible fiber content of plant raw materials, particularly in the context of animal nutrition, biomass valorization, or quality control of botanical extracts.

Objectives of the analysis

Lignin measurement allows us to:

• Characterizing the digestibility of fibers in forages and plant by-products
• Determine the proportion of non-assimilable plant matter in animal feed
• Assessing the quality of biomass for the production of bioenergy or bio-based materials
• Standardizing plant extracts in herbal medicine or nutraceuticals

This parameter is essential for animal feed manufacturers, natural supplement manufacturers and biomaterial producers.

Available analytical methods

Van Soest Method (ADL – Acid Detergent Lignin)

The ADL method is the most commonly used in animal nutrition. It is based on the sequential extraction of different fibrous fractions: NDF (Neutral Detergent Fiber), ADF (Acid Detergent Fiber), and then lignin. It allows for a standardized assessment of residual lignin.

Gravimetry after acid hydrolysis

This method involves isolating crude lignin by acid hydrolysis, followed by gravimetric analysis. It is recommended for processed matrices, those rich in cellulose, or complex extracts.

HPLC

High-performance liquid chromatography (HPLC) analysis is used in some cases to quantify specific lignin subunits or to track structural changes in purified extracts.

Affected matrices

Lignin analysis is applicable to many matrices:

• Raw forage (silage, alfalfa, corn, hay)
• Plant-based raw materials for animal feed
• Plant extracts (tinctures, powders, supplements)
• Lignocellulosic biomass (wood chips, plant waste, agricultural residues)
• Finished products containing complex fibers

Industrial interest

In animal feed, lignin content is a limiting factor in digestibility. Accurate measurement allows for the formulation of fiber-balanced rations. In bioprocesses, it allows for the prediction of energy yields or yields of by-products from biomass.

Lignin analysis is also often combined with complementary measurements such as cellulose, NDF/ADF fibers, or crude ash. See, for example, the fact sheet on the measurement of total, insoluble, and soluble dietary fiber.

Why go through YesWeLab?

YesWeLab provides you with a network of expert laboratories specializing in nutritional and plant-based analyses. Most of our partners are ISO 17025 certified and use methods validated according to AFNOR or Van Soest standards. We support you at every stage:

• Selecting the method best suited to your sample
• Logistics tracking and traceability via our digital platform
• Rapid feedback of results

Request your quote

Do you want to measure lignin in your samples or include this analysis in a complete fiber assessment? Contact our team through the YesWeLab platform. We will direct you to the laboratory best suited to your requirements and deadlines.