Hordenine (phenylethylamine) dosage

Hordenine is an alkaloid belonging to the phenylethylamine family, naturally occurring in cereals, particularly barley. Known for its stimulant properties, it is used in plant extracts, nutritional supplements, energy formulations, and sports nutrition products. Its dosage is essential for ensuring the quality, safety, and regulatory compliance of products containing this molecule.

In the nutraceutical sector, hordenine is valued for its effects on alertness, thermogenesis, and energy metabolism. Its analysis is essential to verify claims, ensure accurate levels, and prevent overdosing. It is also a quality marker in certain plant-based raw materials used in herbal medicine, such as barley extracts, sprouted stems, and standardized botanical blends.

Why analyze hordenine?

Hordenine analysis addresses several industrial needs:

• Quality control of plant extracts rich in phenylethylamines
• Standardization of raw materials for formulation
• Checking the content of energy supplements or pre-workouts
• Compliance analysis with regard to potential regulatory restrictions
• Detection of the presence of hordenine in fermented or barley-based foods

Since hordenine is a relatively sensitive molecule and is present at varying concentrations depending on the extraction processes, its analytical measurement ensures the stability and reproducibility of batches.

Available analytical methods

Hordenine levels are determined by high-performance liquid chromatography or mass spectrometry:

HPLC is
a technique suitable for standardized plant matrices and supplements. It allows for precise separation of alkaloids, guaranteeing reproducible quantification even in complex mixtures.

LC-MS or LC-MS/MS
is the reference method for detecting hordenine at very low concentrations. It offers excellent sensitivity and maximum selectivity, clearly distinguishing hordenine from other phenylethylamines or similar structural molecules. It is the preferred technique for challenging matrices, highly concentrated supplements, or products intended for sports nutrition.

YesWeLab systematically assists its clients in choosing the most appropriate technique according to the matrix, the acceptable level of uncertainty and regulatory constraints.

Analyzable matrices

• plant extracts (barley, grasses, plants rich in alkaloids)
• standardized powders and extracts
• dietary supplements (capsules, tablets, liquid shots)
• sports nutrition products
• foods or technical ingredients derived from fermentation

Some matrices require prior hydrolysis or specific extraction protocols to ensure optimal recovery of the molecule.

Internal linking to other relevant analyses

Several additional services from the YesWeLab catalogue can be combined with hordenine analysis for a complete characterization of extracts or supplements:

LC-MS analysis:
Reference method for identifying and quantifying bioactive alkaloids, polyphenols and metabolites.

HPLC chromatography:
A technique suitable for the standardization of plants and nutraceutical extracts.

Analysis of total polyphenols
Often carried out as a complement to characterize bioactives in plant extracts.

Spectrophotometry:
A useful method for antioxidant parameters or for coupled analyses in complex extracts.

Why entrust this dosage to YesWeLab?

YesWeLab selects the most qualified laboratory for each request, based on the matrix, methodology, and client requirements. We work with an expert network that includes laboratories specializing in chromatography and nutraceutical analysis, many of which are ISO 17025 accredited. Our platform centralizes all analyses, ensures secure sample shipments, and allows for seamless results tracking. Since 2020, numerous supplement brands, ingredient distributors, and formulators have entrusted us with the quality control of their extracts and active ingredients.