Echinacoside (hydroxycinnamic acid) dosage

Echinacoside is a polyphenolic compound belonging to the hydroxycinnamic acid family, primarily found in species of the genus Echinacea (notably Echinacea angustifolia and Echinacea purpurea ). This secondary metabolite possesses potent antioxidant, anti-inflammatory, and immunomodulatory properties, which explain its widespread use in natural health products and dietary supplements.

The dosage of echinacoside is a key indicator of the quality and standardization of echinacea extracts. It guarantees the content of active ingredients, ensures regulatory compliance of products, and validates the consistency of production batches.

Advantages of analytical measurement

Echinacoside dosage is essential for:

• Control the active ingredient content of echinacea extracts and ensure their standardization.
• Verify the botanical authenticity of the plant raw materials.
• Evaluate the overall antioxidant potential of phytotherapeutic products.
• Ensure that food supplements comply with the manufacturer's specifications.
• To study the stability of echinacoside during storage or processing.

This analysis is particularly recommended for laboratories and manufacturers developing products with immune, antioxidant or anti-fatigue targets.

Analytical methods used

At YesWeLab, echinacoside is analyzed according to validated chromatographic protocols, guaranteeing accurate identification and quantification of the compound.

High-performance liquid chromatography (HPLC-DAD)
The HPLC-DAD (High Performance Liquid Chromatography – Diode Array Detection) method allows for the specific separation of phenolic acids present in echinacea extracts. UV detection at specific wavelengths (320-330 nm) ensures reliable and reproducible measurements.

Liquid chromatography coupled with mass spectrometry (LC-MS or LC-MS/MS)
The LC-MS technique offers increased sensitivity and unambiguous identification, even in complex matrices. It allows the differentiation of echinacoside from other co-extracted phenolic compounds and provides precise quantitative data, particularly at low concentrations.

All analyses are carried out in partner laboratories accredited to ISO 17025 and compliant with good analytical practices (GLP).

Industrial and sectoral applications

Nutraceutical:
Echinacoside is a quality marker for echinacea-based food supplements used to support the immune system and protect against oxidative stress. Its precise dosage ensures a consistent concentration of active ingredients and contributes to the traceability of the formulations.

herbal medicine
, the echinacoside content in medicinal plant extracts helps to verify the botanical identity and quality of preparations (hydroalcoholic extracts, dry powders, mother tinctures). It also helps to assess the synergy between caffeic acid derivatives, such as cynarin or cichoric acid.

Natural cosmetics:
Some anti-aging or soothing formulations contain echinacea extracts for their antioxidant and restorative effects. Analysis of echinacoside ensures the consistency and stability of the active ingredient in creams or serums.

Research and development
In phytochemical research, this assay is used to characterize the phenolic profiles of different echinacea species, evaluate the effects of extraction processes and study the correlations between composition and biological activity.

Affected matrices

• Standardized dry extracts of echinacea
• Plant powders and capsules
• Hydroalcoholic solutions and dyes
• Liquid products for immune function
• Antioxidant creams and serums

Recommended Related Analyses

For a complete characterization of the phenolic profile of plant extracts, YesWeLab recommends combining the following:
Caffeic acid assay – another hydroxycinnamic acid present in Echinacea .
Cynarin assay – a polyphenolic marker in artichoke extracts, often associated with echinacoside.
Ferulic acid assay – to assess the overall antioxidant potential of the extracts.
Total polyphenol assay – for a comprehensive view of the phenolic richness of natural products.

Why choose YesWeLab?

YesWeLab relies on a network of ISO 17025 accredited partner laboratories specializing in chromatographic analysis of plant extracts. Thanks to our cutting-edge methods and scientific support, we guarantee reliable, compliant, and actionable results for the development, certification, or market launch of your products.

Our digital platform allows you to centralize your requests, track your samples in real time and consult your analytical reports in a simple and secure way.