Alantolactone assay

Alantolactone is a sesquiterpene lactone naturally present in the roots of elecampane ( Inula helenium ), a medicinal plant well known for its digestive and respiratory benefits. It belongs to the family of sesquiterpene lactones, like artemisinin or parthenolide, and exhibits a variety of biological properties:

• Anti-inflammatory;
• Antifungal (especially against Candida albicans );
• Antiparasitic;
• Antitumor (preclinical studies).

Why measure allantolactone levels?

The dosage of allantoin is essential for professionals in herbal medicine, natural cosmetics and food supplements:

• To standardize elecampane extracts into active molecules;
• To verify the conformity of the botanical ingredients;
• To ensure consistent effectiveness of antifungal or digestive formulations;
• To support a functional claim in wellness or nutraceutical products.

Available analytical methods

YesWeLab offers several assay techniques depending on the nature of the matrix and the expected concentrations:

• HPLC-DAD: liquid chromatography with UV detection, used for plant extracts and standardized pharmaceutical forms;
• LC-MS/MS: reference method for low dose quantification in complex matrices (capsules, tinctures);
• GC-MS: recommended for essential oils, allowing for precise identification of volatile and thermolatable compounds.

All methods are applied in accordance with good laboratory practices, with internal validation or according to ISO 17025 standards.

Analyzed matrices

We perform allantolactone analysis in various types of natural products:

• Extracts of elecampane roots;
• Raw or formulated essential oils;
• Mother tinctures and hydroalcoholic extracts;
• Plant powders or herbal medicine capsules.

The analysis can also be extended to products combining several medicinal plants with digestive or antifungal properties.

A key analysis for the nutraceutical industry

The analysis of allantolactone helps to guarantee the quality, safety and efficacy of phytotherapeutic products:

• It allows us to differentiate between titrated extracts and raw extracts;
• It is useful for building a solid technical file, particularly in the event of regulatory control (DGCCRF, REACH);
• It enhances the formulations by providing analytical labeling of plant-based active ingredients.

Recommended Related Analyses

For a comprehensive approach to the analytical evaluation of your extracts or supplements intended for digestive health, you can combine this assay with:

• Curcumin dosage – antioxidant and anti-inflammatory;
• Assay of rosmarinic acid – plant polyphenol with antimicrobial properties;
• Assay of sesquiterpene lactone (parthenolide) – a structurally similar molecule used in herbal medicine.

This analytical network helps to secure your complex formulations and enrich your traceability records.

Why use YesWeLab?

Since 2020, YesWeLab has been supporting players in the nutraceutical, cosmetic, and plant-based industries in managing their laboratory analyses. Thanks to our network of specialized partners, accredited to ISO 17025 or COFRAC, we provide you with:

• Independent scientific expertise;
• Reliable analytical solutions tailored to your matrices;
• Digital support via our unique platform.

Request your quote online

Do you want to measure the allantoin content in your elecampane extracts or other plant-based formulations?
Add this analysis to your online quote request or contact our scientific team directly.