Adenosine assay

Adenosine is a purine nucleoside naturally present in plant and animal cells. Composed of adenine and ribose, it plays a central role in energy metabolism (ATP/ADP/AMP), cell signaling, and physiological regulation. It is involved in, among other things:

• Regulation of sleep and wakefulness (calming effect on the central nervous system);
• The slowing of the heart rate;
• Modulation of inflammatory responses;
• The mechanisms of vasodilation and neuronal protection.

In the nutraceutical sector, adenosine is sought after for its neuroactive effects and its potential role in recovery, stress management, and overall well-being. It is also used as a quality marker in plant extracts and functional products.

Methods for measuring adenosine

Adenosine analysis requires sensitive and specific techniques. At YesWeLab, we primarily use:

• HPLC-DAD (high-performance liquid chromatography with UV detection) – suitable for simple or standardized matrices;
• LC-MS / LC-MS/MS (chromatography coupled with mass spectrometry) – recommended for complex matrices or low concentrations.

The choice of method depends on the matrix being analyzed (plant, supplement, infusion) and the level of quantification required.

Analyzed matrices

Adenosine testing is available for a wide range of natural and processed products:

• Plant extracts (seeds, roots, leaves, medicinal mushrooms);
• Dietary supplements based on adaptogenic, tonic or calming plants;
• Functional infusions and herbal teas (valerian, ginseng, ashwagandha, cordyceps, etc.);
• Fortified nutritional products – powders, drinks, capsules.

Industrial Applications and Challenges

Adenosine is an interesting biomarker in many contexts:

• Quality control of plant extracts intended for relaxing, anti-stress or cognitive uses;
• Standardization of neuroactive or nootropic food supplements;
• Monitoring the concentration of active ingredients in complex formulations;
• Research and development for the formulation of innovative products targeting mental well-being.

It is also used in some veterinary products or premium pet food, particularly for joint comfort or stress management.

Recommended Related Analyses

For a complete evaluation of your extracts or formulations intended for neuroactive effects, it is advisable to combine adenosine measurement with other compounds or tests:

• Caffeine dosage – stimulant antagonist of adenosine receptors;
• Dosage of theobromine – an alkaloid with complementary effects in the nervous system;
• Trigonelline dosage – a molecule present in coffee, often associated with adenosine;
• Tryptamine dosage – another neuroactive precursor involved in mood modulation.

These analyses allow you to evaluate the synergy of the bioactive compounds present in your formulations and to optimize their scientific and marketing positioning.

Why choose YesWeLab?

YesWeLab offers a complete solution for adenosine assay, tailored to your regulatory, marketing, or R&D objectives. Thanks to our network of rigorously selected and accredited partner laboratories (ISO 17025, COFRAC, etc.), we guarantee:

• Reliable and sensitive analytical techniques (HPLC, LC-MS);
• An adaptation to your specific matrices;
• Fast and personalized support via our digital platform;
• Recognized expertise in nutritional, functional and plant-based analyses.

Need to quantify adenosine in your extracts or supplements?

Add this test to your online quote request or contact our scientific team to discuss your specifications.