Coliform determination (high sensitivity petrifilm)

The Petrifilm method for coliform counts is a rapid, reliable, and standardized technique for enumerating these indicator bacteria in various types of matrices. Coliforms are microorganisms frequently used as indicators of hygiene and microbiological contamination, particularly in the food industry and the production of biological ingredients. This method relies on the use of ready-to-use gelled plates, enabling precise and reproducible quantification after incubation.

Principle and operation of the method

The high-sensitivity Petrifilm test is based on counting coliform colonies cultured on a nutrient-enriched gel medium with chromogenic indicators.
The sample, diluted according to a protocol adapted to its matrix, is deposited onto the Petrifilm plate. After incubation at a controlled temperature, typical colonies are visible and counted directly. This approach significantly reduces handling and analysis time compared to traditional culture methods.

For confirmatory or complementary analyses, the method can be combined with quantitative PCR or next-generation sequencing (NGS) techniques to precisely identify the strains present. These molecular approaches enhance the traceability and reliability of results, particularly for products with stringent health requirements.

Analytical performance and technical strengths

The high-sensitivity Petrifilm test is renowned for its simplicity, speed, and high sensitivity. It detects low levels of contamination while ensuring excellent repeatability of results. This method is compatible with a wide range of matrices: powders, plant extracts, fermented products, milk, beverages, nutritional ingredients, and water.

The analysis can be complemented by other services from the YesWeLab catalogue such as yeast and mold enumeration , Escherichia coli assay, or global microbiological control to obtain a complete microbiological quality profile of a product.

Applications and areas of use

Coliform measurement is essential in many industrial sectors:

• Human food : control of production hygiene and microbiological safety of foodstuffs, particularly in fermented, dairy and plant products.
• Animal nutrition : verification of the microbiological quality of animal feed, premix and functional ingredients.
• Nutraceuticals : quality control of extracts and food supplements of natural origin to guarantee the absence of contamination.
• Plants and plant extracts : microbiological monitoring of powders, essential oils or botanical extracts, in conjunction with other analyses such as the measurement of antioxidants or the search for heavy metals .

These controls make it possible to verify compliance with regulatory requirements and to ensure the stability of products throughout their life cycle.

The advantages of the YesWeLab service

YesWeLab relies on a network of partner laboratories specializing in microbiology, rigorously selected according to quality and compliance criteria (ISO 17025, COFRAC). Each request is analyzed to be directed to the most suitable laboratory based on the matrix, method, and required sensitivity level.

Thanks to its digital analysis platform, YesWeLab simplifies the management of your microbiological tests: instant quotes, sample submission, analysis tracking, and results delivery. Since 2020, numerous manufacturers, distributors, and engineering firms have trusted us to manage their health and safety controls.

For any specific needs in coliform assay using the high-sensitivity Petrifilm method, contact our scientific team to obtain tailored support and a personalized quote.