Hederagenin dosage

Hederagenin is a triterpene saponin naturally present in ivy (Hedera helix). This molecule is widely used in the nutraceutical, phytotherapeutic, and cosmetic industries for its anti-inflammatory, expectorant, healing, and antimicrobial properties. Its concentration is essential for verifying the conformity of plant raw materials, controlling the quality of standardized extracts, and ensuring the safety of complex formulations.

In extracts of ivy or related plants, hederagenin is found in free or conjugated form (saponosides). Accurate quantification is therefore a key step in quality control, particularly for manufacturers of active ingredients, formulators of food supplements, and R&D laboratories involved in the development of plant-based active ingredients.

Analytical interest and industrial applications

The measurement of hederagenin helps to meet several industrial needs:

• Quality control of plant extracts
• Standardization of active ingredients in food supplements
• Verification of the conformity of the raw material according to the specifications
• Securing batches of semi-solid or liquid cosmetic products
• Optimization of formulations incorporating standardized extracts
• Stability and compatibility studies in finished products

This analysis is used in various contexts: supplier audits, internal certification, development of new extracts, optimization of natural processes, or identification of variability problems in plant supply chains.

Available analytical methods

Hederagenin levels are primarily measured by liquid chromatography:

HPLC-DAD
is a method commonly used for the quantification of plant compounds. It allows for precise separation of saponins and reliable quantification from a certified standard.

LC-MS or LC-MS/MS
is the reference method for complex or highly colored matrices. It offers high specificity, excellent sensitivity, and allows differentiation of similar structures.

Depending on regulatory or R&D requirements, YesWeLab selects the most suitable method based on the matrix, target concentration, or potential interferences. This analysis can be combined with other saponin or alkaloid assays, particularly when the extract is multi-active.

Matrices that can be analyzed

• hydroalcoholic dry extracts
• vegetable powders
• Tinctures and macerates
• nutraceutical capsules and tablets
• cosmetic creams and gels
• syrupy solutions or technical liquids

For complex formulations, pretreatment (extraction, SPE purification) may be required to ensure reliable quantification.

Internal linking to other relevant analyses

Hederagenin assay is often part of a broader analytical panel focusing on plant-based active ingredients. Several services from the YesWeLab catalog may be relevant to complement this analysis:

HPLC analysis:
A method commonly used for the determination of polyphenols, alkaloids, saponins, and vitamins.

Berberine dosage:
Relevant analysis for multi-active plant extracts containing several alkaloids.

Palmatin assay:
Analytical supplement for Asian or metabolic extracts.

Curcumin dosage:
Interesting for nutraceutical players working on multi-active anti-inflammatory complexes.

LC-MS/MS analysis
recommended for complex matrices, low concentrations or extracts that are difficult to purify.

Why go through YesWeLab for this analysis?

YesWeLab selects the most suitable laboratory based on the matrix, the desired method (HPLC or LC-MS/MS), the objective of the analysis, and regulatory constraints. Our network of over 200 partner laboratories includes ISO 17025 and COFRAC accredited facilities specializing in the analysis of plants and nutraceutical products.

The YesWeLab platform allows you to centralize your requests, track your samples in real time, and easily retrieve your results. Since 2020, numerous manufacturers, formulators, and R&D laboratories have relied on our services to ensure the quality of their plant-based products.