HDA dosage

HDA (10-hydroxy-2-decenoic acid, often abbreviated as 10-HDA) analysis is essential for professionals in the beekeeping, nutrition, and dietary supplement sectors. This specific unsaturated fatty acid is naturally present in royal jelly, where it plays a fundamental role as an indicator of freshness, purity, and quality.

The quantification of 10-HDA is therefore used as a criterion for standardizing and valuing bee products. Its measurement allows manufacturers, distributors, and laboratories to guarantee the conformity of their products with international standards and market expectations.

Understanding the HDA and its importance

10-HDA is a bioactive fatty acid characteristic of royal jelly. Its concentration is often considered the main quality marker of this natural product.

The roles and interests associated with HDA testing include:

• Verification of the authenticity of royal jelly (prevention of fraud and falsification)
• Determining the freshness and stability of the product
• Standardization of batches intended for food supplements
• Evaluation of the nutritional and functional value of finished products

Some regulations and quality standards (particularly in Asia and Europe) set minimum levels of 10-HDA for a product to be marketed as pure royal jelly.

Analytical methods for HDA assay

The HDA assay is performed by HPLC (high-performance liquid chromatography) , a reference technique for the analysis of organic acids and bioactive compounds.

This method has several advantages:

• High precision and reproducibility
• Ability to analyze complex matrices such as raw or processed royal jelly
• Speed ​​and reliability for routine quality control
• Adaptability to different types of samples (food products, capsules, solutions, combination formulations)

The use of HPLC ensures rigorous monitoring of royal jelly quality while meeting international standards.

Sectors affected by HDA testing

10-HDA analysis applies to several areas:

• Beekeeping : quality control of raw and standardized royal jelly.
• Food supplements : verification of formulations based on royal jelly in powder, capsule or liquid solutions.
• Nutrition : development of functional products enriched with royal jelly.
• Scientific research : study of the bioactive properties and natural variations of 10-HDA.

Typically analyzed matrices

The 10-HDA assay can be applied to different matrices:

• Raw royal jelly
• Freeze-dried or powdered royal jelly
• Dietary supplements (capsules, tablets, liquid solutions)
• Food products enriched with royal jelly
• Beekeeping mixtures containing royal jelly

Quality and compliance issues

The market value of royal jelly depends largely on its 10-HDA content. The implications of this analysis are numerous:

• Authenticity and traceability : preventing fraud and guaranteeing product purity.
• Regulatory compliance : meeting the standards imposed by export authorities and markets.
• Standardization : offering consumers a homogeneous product of consistent quality.
• Commercial valuation : highlighting a high 10-HDA rate as a differentiating factor.

YesWeLab support for your royal jelly analyses

At YesWeLab, we work with a network of partner laboratories specializing in physicochemical and nutritional analyses. Our partners, certified and accredited (ISO 17025, COFRAC), have validated methods for the precise measurement of 10-HDA.

Our digital platform allows you to:

• Submit your samples online quickly
• Track your requests and analyses in real time
• Receive reliable and secure results that meet regulatory and commercial expectations

Since 2020, many manufacturers, distributors and players in the beekeeping sector have entrusted us with their analyses to secure their products and strengthen consumer confidence.

Related analyses

• Fatty acid dosage 
• Total polyphenol profiling
• Sugar analysis (honey, bee products)
• Coumarin dosage
• Vitamin C dosage