Glutathione dosage

Glutathione (CAS No. 70-18-8) is a antioxidant tripeptide composed of glutamate, cysteine, and glycine . Present in all plant and animal cells, it plays a key role in defense against oxidative stress , cellular detoxification , and the maintenance of intracellular redox potential .

Glutathione testing allows for the quantification of the concentration of the reduced form (GSH) and, if necessary, the oxidized form (GSSG) . This analysis is essential for evaluating the quality, efficacy, and stability of products with antioxidant or detoxifying properties, whether they are natural extracts, dietary supplements, or nutraceutical formulations.

Analytical methods: HPLC and LC-MS

Since glutathione is a heat-sensitive and reactive molecule, its measurement requires precise and specific methods:

• HPLC (high-performance liquid chromatography) : used after derivatization to stabilize glutathione and allow selective detection.
• LC-MS (liquid chromatography coupled with mass spectrometry) : a highly sensitive and specific method for distinguishing GSH and GSSG forms and detecting very low concentrations.

These approaches guarantee reliable and reproducible quantification of glutathione, in accordance with the requirements of quality control and nutraceutical R&D .

Industrial applications

Glutathione testing is applicable to many areas:

• Nutraceuticals and food supplements : control of natural antioxidant content and validation of functional claims.
• Agri-food : evaluation of the antioxidant activity of plant extracts, juices or fortified products.
• Phytotherapy and medicinal plants : characterization of extracts rich in protective sulfur compounds.
• Research and innovation : studies on cellular metabolism, the response to oxidative stress and the bioaccessibility of nutrients.

For a complete analysis of the antioxidant profile, glutathione measurement can be combined with glutathione peroxidase (GPX) measurement or superoxide dismutase (SOD) measurement .

Affected matrices

Our partner laboratories perform glutathione assays on various matrices:

• Plant extracts (medicinal plants, fruits, vegetables)
• Dietary supplements in the form of capsules, powders or functional drinks
• Food products enriched with antioxidants
• Biological samples or plant fermentations

Depending on the matrix, the analytical protocol may include a specific preparation step (aqueous extraction, filtration, neutralization) to ensure optimal measurement of free and total glutathione .

YesWeLab expertise

At YesWeLab , we collaborate with an international network of more than 200 partner laboratories , located in France, Europe and internationally , and selected for their technical expertise and their ISO 17025 and COFRAC accreditation .

Our scientific team will guide you in choosing the most suitable method , selecting the specialized laboratory , and providing comprehensive support for your analyses . Thanks to our intuitive digital platform , you can centralize your requests , track your samples , and obtain your results online .

Since 2020, many manufacturers, distributors and design offices have entrusted us with their analyses to guarantee the reliability and conformity of their products.

Order a glutathione analysis

Do you want to measure the glutathione content in your extracts, formulations or antioxidant products?
Submit your request via our platform or contact our scientific team for personalized support .

→ Add to quote now to get a quick and accurate quote.