Free theanine (amino acid) dosage

L-theanine is a non-protein amino acid naturally present in green tea (Camellia sinensis). Particularly sought after for its relaxing effects without causing drowsiness, it is the subject of growing interest in the nutraceutical field. Its activity is linked to a modulation of neurotransmission, notably via GABA, dopamine, and serotonin. It is also known to promote concentration, reduce stress, and improve the quality of mental attention.

Free theanine measurement is essential for manufacturers who want to ensure the quality, compliance, and efficacy of their green tea or functional ingredient products. It also helps validate the claim related to L-theanine concentration in formulations intended for mental well-being or cognitive nutrition.

Available analysis methods: HPLC or LC-MS

L-theanine analysis is typically performed by high-performance liquid chromatography (HPLC) or liquid-phase mass spectrometry (LC-MS). In both cases, a derivatization step is often implemented to improve the detection and separation of this amino acid in complex matrices.

• HPLC-DAD : suitable for concentrated or purified plant extracts.
• LC-MS/MS : recommended for rich matrices (beverages, multi-active powders) or for low concentrations, with very good analytical specificity.

Commonly analyzed matrices

The dosage of L-theanine is applicable to several types of products:

• Standardized green tea extracts
• Fortified functional drinks
• Food supplements (capsules, tablets, soluble powders)
• Multi-ingredient formulations for neurocognitive purposes

Why analyze L-theanine?

• Quality control of raw materials or titrated extracts
• Standardization of green tea-based formulations or natural nootropics
• Regulatory validation in the case of claims regarding relaxing or cognitive effects
• Product development for formulas for mental health, sleep or concentration

Integration into nutraceutical projects

Increasingly integrated into nutritional complexes dedicated to stress, concentration or mental recovery, L-theanine is also combined with other active ingredients such as caffeine, rhodiola or magnesium. Its precise quantification allows for better formulation control, ensuring consistency between batches and justifying the desired effects in technical or marketing files.

YesWeLab Analytical Services

At YesWeLab, we provide you with advanced technical expertise and a network of accredited partner laboratories (ISO 17025, COFRAC, etc.) for the determination of L-theanine and other functional amino acids. Analytical methods are chosen according to the complexity of your matrices, the objectives of your analysis (development, control, compliance), and your regulatory requirements.

Thanks to our digital platform, you can easily:

• Compare available methods
• Order your analyses
• Track your samples
• Access your results securely

Our scientific team is at your disposal to adapt the methods to your specific constraints and ensure a rapid and reliable response.

Useful links to other related analytical services

• Glutamine dosage : another amino acid involved in cellular nutrition.
• Glycine dosage : for neuroprotective and relaxant formulations.
• Caffeine Dosage : Often combined with theanine in nootropic formulas.
• Flavonoid analysis : to characterize antioxidant-rich tea extracts.

Need personalized support?

Since 2020, dozens of players in the nutraceutical sector have entrusted us with the management of their analyses to ensure the reliability of their products. Trust YesWeLab for standardization, compliance, and analytical innovation in your projects.

Contact our team to submit a specific need or obtain a personalized quote as soon as possible.