Frangulin A and frangulin B dosage

Frangulins are anthraquinone glycosides naturally present in the bark of alder buckthorn (Rhamnus frangula). They constitute essential chemical markers in the evaluation of the quality and efficacy of alder buckthorn extracts, traditionally used as mild laxatives in herbal medicine.

Their laboratory testing ensures regulatory compliance, standardization of plant extracts and control of the content of active ingredients, in order to guarantee both the safety and efficacy of the finished products.

Importance of the franguline dosage

Frangulin analysis is of major importance to manufacturers of herbal medicine, nutraceuticals, and dietary supplements. These molecules are responsible for the desired biological activity in buckthorn-based preparations.

Precise dosage is necessary for:

• control the content of active ingredients and standardize the extracts
• to ensure compliance with the pharmacopoeia and regulatory recommendations
• to ensure safe use by avoiding overdoses
• to scientifically evaluate the use of buckthorn extracts intended for the nutraceutical market

The quantitative determination of frangulins is therefore a central element of quality control in the production chain of plant extracts.

Available analytical methods

Frangulin analysis relies on high-precision chromatographic techniques:

• HPLC-DAD (high-performance liquid chromatography with diode array detection), which allows for fine separation and reliable quantification of anthraquinones in complex matrices.
• LC-MS (liquid chromatography coupled with mass spectrometry), which offers enhanced sensitivity and specificity to confirm the molecular identity of frangulins and simultaneously detect other anthraquinones present in buckthorn.

These analytical techniques are suitable for both raw materials and finished products, guaranteeing complete traceability and optimal reliability of results.

Analyzed matrices

Frangulines' analyses mainly concern:

• standardized plant extracts of buckthorn
• powders made from dried bark
• preparations based on buckthorn used in herbal medicine and nutraceuticals

The dosage can be carried out in both simple products and complex formulations containing several plants.

Industrial applications

Assessing frangulin content is essential for manufacturers of food supplements and herbal products. It allows them to support market entry with reliable analytical data that meets regulatory requirements.

This analysis also meets the needs of distributors and research offices responsible for verifying batch quality, assessing product stability and meeting the requirements of health authorities.

Additional analyses available

For a complete characterization of plant extracts, YesWeLab offers other assays for anthraquinone and flavonoid compounds. Among the analyses available in our catalog:

Emodin (anthraquinone) assay,
aloin (anthraquinone) assay,
chrysophanol (anthraquinone) assay,
quercetin (flavonoid) assay,
rutin (flavonoid) assay

These services make it possible to complete the analytical profile of plant extracts, to optimize standardization and to strengthen the scientific value of products on the market.

YesWeLab expertise

YesWeLab provides access to its network of rigorously selected partner laboratories, most of which are ISO 17025 and COFRAC certified and accredited. Each project is assigned to the most suitable laboratory based on the matrix, the required methodology, and regulatory requirements.

Since 2020, our digital platform has simplified analysis management for numerous manufacturers and distributors. It allows for the centralization of requests, ensures real-time monitoring, and delivers fast and reliable results.

Our scientific team supports each client with responsiveness and precision, in order to meet specific needs in terms of quality control and regulations.