Vicenin-2 (apigenin-6, 8-diglucoside) dosage

The measurement of vicenin-2 , also known as apigenin-6,8-diglucoside , allows for the quantification of this diglucosylated flavonoid naturally present in several medicinal plants such as tulsi (holy basil) , canary grass, and certain grasses. This compound is attracting increasing interest in nutraceuticals for its antioxidant, neuroprotective, anti-inflammatory, and anti-stress properties .

The analysis of vicenin-2 makes it possible to ensure the standardization and valorization of plant extracts used in food supplements, functional drinks and wellness formulations.

Quantification is performed by high-performance liquid chromatography (HPLC-DAD) or by liquid chromatography coupled with mass spectrometry (LC-MS), two reference techniques for the identification and quantification of glycosylated flavonoids.

Principle and operation of the method

The sample (plant extract, powder, functional drink or capsule) is first subjected to a selective extraction of flavonoids using polar solvents.

• In HPLC-DAD , separation is based on the polarity and size of the molecules. Diode array detection (DAD) allows tracking of vicenin-2 at its specific wavelength (approximately 330 nm).
• In LC-MS , the molecule is identified according to its mass/charge ratio (m/z), offering precise structural identification even in complex matrices.

These approaches ensure reliable and reproducible quantification, essential for studies of formulation, stability and quality of nutraceutical products.

Analytical performance and related analyses

HPLC and LC-MS methods offer:

• high sensitivity enabling detection at trace levels,
• high selectivity for distinguishing vicenin-2 from other glycosylated flavones,
• optimal reproducibility for batch-to-batch controls.

Vicenin-2 measurement can be combined with other analyses to characterize the polyphenolic richness of plant extracts, including:

• total flavonoid dosage,
• luteolin dosage ,
• apigenin dosage,
• the measurement of total polyphenols,
  in order to draw up a complete profile of antioxidant compounds.

Applications and areas of use

Vicenin-2 assay is applicable in several fields:

• Nutraceuticals : standardization of plant extracts rich in flavonoids for anti-stress, neuroprotective or antioxidant formulations.
• Phytotherapy : quality control of tulsi or canary seed extracts used for their soothing and adaptogenic effects.
• Functional drinks : monitoring of vicenin-2 content in herbal drinks intended for wellness or recovery.
• Research and development : identification of new plant extracts rich in glycosylated flavones and evaluation of their bioavailability.

This analysis contributes to the scientific and commercial valorization of natural ingredients by guaranteeing their authenticity and stability.

The advantages of the YesWeLab service

YesWeLab collaborates with a network of partner laboratories accredited to ISO 17025 and COFRAC standards, specializing in the analysis of polyphenolic compounds and flavonoids. Each sample is entrusted to the most suitable laboratory based on the matrix, the method, and regulatory requirements.

Thanks to its digital analysis platform, YesWeLab simplifies the management of your analytical projects: quote requests, online tracking, and rapid results delivery. Since 2020, numerous manufacturers, formulators, and research laboratories have trusted us to characterize their plant extracts and dietary supplements.

For any specific request regarding vicenin-2 assay, contact our scientific team to obtain a personalized quote and tailored technical support.