Dosage of verbascoside (acteoside)

The dosage of verbascoside , also known as acteoside , allows the quantification of this major phenolic compound present in many plant species such as Plantago lanceolata , Verbascum thapsus or Olea europaea .
This phenylpropanoid heteroside antioxidant, anti-inflammatory, antimicrobial and neuroprotective properties , making it an essential biomarker in the quality assessment of plant extracts and nutraceutical or cosmetic formulations.

The assay is performed by high-performance liquid chromatography (HPLC) diode-array detection or mass spectrometry (LC-MS/MS) for accurate and sensitive compound identification.

Analytical methods and protocols

The method is based on selective extraction of phenolic compounds, followed by chromatographic separation on a C18 column.
HPLC -DAD allows detection of verbascoside at a specific wavelength (typically 330 nm), while LC-MS/MS offers finer quantification, particularly in complex matrices.

The parameters measured include:

• The verbascoside rate (mg/g or %),
• The complete chromatographic profile (other associated phenylpropanoids),
• The purity of the extract ,
• And the stability of the compound within the formulation.

The methods can be adapted according to the European Pharmacopoeia or USP for products for nutraceutical or cosmetic use.

Industrial and scientific applications

The dosage of verbascoside is an essential tool for players in the sectors:

• Nutraceuticals : standardization of plant extracts rich in polyphenols, quality control of antioxidant or anti-inflammatory formulations.
• Cosmetics : validation of the soothing, protective and anti-aging activity of natural active ingredients.
• Phytotherapy : identification and quantification of acteoside as a marker molecule in extracts of Plantago , Verbascum or Olea .
• Food industry : valorization of functional ingredients from medicinal plants.

The results make it possible to validate the conformity of raw materials , to guarantee the reproducibility of batches and to justify functional or regulatory claims .

Analyzed matrices

• Standardized plant extracts (leaves, roots, flowers),
• Herbal food supplements,
• Natural cosmetic formulations,
• Phytotherapeutic raw materials,
• Products enriched with polyphenols.

Further analyses recommended

For a complete characterization of phenolic compounds and better traceability, YesWeLab recommends combining this assay with:

• Chlorogenic assay for monitoring correlated antioxidant compounds,
• Luteolin assay for the evaluation of associated flavonoids,
• Verbenoside assay for comparison of active ingredient profiles in Verbenaceae extracts,
• Fraxin dosage to complement the coumarin profile of extracts.

YesWeLab expertise

YesWeLab provides a network of ISO 17025 and COFRAC accredited laboratories , experts in chromatographic analyses and natural phytocompounds .

Thanks to the YesWeLab digital platform , you can:

• Order your analyses online,
• Track your samples and get your complete analysis reports,