Dosage of verbascoside (acteoside)

Verbascoside , also known as acteoside measured to quantify this major phenolic compound found in many plant species such as Plantago lanceolata , Verbascum thapsus , and Olea europaea .
This phenylpropanoid glycoside antioxidant, anti-inflammatory, antimicrobial, and neuroprotective properties , making it an essential biomarker for evaluating the quality of plant extracts and nutraceutical or cosmetic formulations.

The assay is performed by high-performance liquid chromatography (HPLC) , often coupled with diode-array detection (DAD) or mass spectrometry (LC-MS/MS) for accurate and sensitive identification of the compound.

Analytical methods and protocols

The method is based on a selective extraction of phenolic compounds, followed by chromatographic separation on a C18 column.
HPLC -DAD allows detection of verbascoside at a specific wavelength (330 nm in general), while LC-MS/MS offers finer quantification, particularly in complex matrices.

The parameters measured include:

• The verbascoside level (mg/g or %),
• The complete chromatographic profile (other associated phenylpropanoids),
• The purity of the extract ,
• And the stability of the compound within the formulation.

The methods can be adapted according to the European Pharmacopoeia or USP for products with nutraceutical or cosmetic use.

Industrial and scientific applications

Verbascoside assay is an essential tool for stakeholders in the following sectors:

• Nutraceuticals : standardization of plant extracts rich in polyphenols, quality control of antioxidant or anti-inflammatory formulations.
• Cosmetics : validation of the soothing, protective and anti-aging activity of natural active ingredients.
• Phytotherapy : identification and quantification of the acteoside as a marker molecule in extracts of Plantago , Verbascum or Olea .
• Agri-food : valorization of functional ingredients derived from medicinal plants.

The results make it possible to validate the conformity of raw materials , to guarantee the reproducibility of batches and to or regulatory claims

Matrices analyzed

• Standardized plant extracts (leaves, roots, flowers),
• Herbal dietary supplements,
• Natural cosmetic formulations,
• Phytotherapeutic raw materials,
• Products enriched with polyphenols.

Further analyses recommended

For complete characterization of phenolic compounds and improved traceability, YesWeLab recommends combining this assay with:

• Chlorogenic assay for monitoring correlated antioxidant compounds,
• Luteolin assay for the evaluation of associated flavonoids,
• Verbenoside assay for comparing active ingredient profiles in verbena extracts,
• Fraxin dosage to complete the coumarin profile of the extracts.

YesWeLab's expertise

YesWeLab provides a network of ISO 17025 and COFRAC accredited laboratories , experts in chromatographic analyses and natural phytocompounds .

Thanks to the YesWeLab digital platform , you can:

• Order your analyses online,
• Track your samples and obtain your complete analysis reports,