Dosage of valepotriates (sesquiterpene iridoids) (valtrate)

The measurement of valepotriates , and more specifically valtrate (CAS No. 18296-44-1), is a key analysis for characterizing extracts of valerian (Valeriana officinalis) and other medicinal plants with calming and relaxing properties. These compounds, belonging to the sesquiterpene iridoid family, are recognized for their role in the biological activity of valerian-based preparations, which are often used in nutraceuticals, herbal medicine, and natural health products.

Principle and importance of the analysis

Valepotriates are unstable esters of iridoids, naturally present in the roots of valerian and some related species. Among them, valtrate , isovaltrate , and didrovaltrate are the main chemical markers sought to evaluate the quality and potency of plant extracts.

Their dosage allows for:

• Control the content of active compounds in valerian extracts or formulated products;
• Standardize raw materials for nutraceutical or pharmaceutical use;
• To guarantee the stability and effectiveness of finished products (capsules, tablets, infusions);
• Verify regulatory compliance and batch consistency in a quality and traceability framework.

Due to their chemical instability (sensitive to heat and light), rigorous handling and storage of samples are necessary to obtain reliable results.

Analytical methods used

Valepotriates are measured by high-performance liquid chromatography (HPLC) or by liquid chromatography coupled with mass spectrometry (LC-MS or LC-MS/MS).

• HPLC-DAD (diode array detection) allows the separation and quantification of different valepotriates in a plant extract.
• LC-MS/MS offers more specific detection, suitable for complex matrices and low concentrations.

Depending on the objectives of the analysis, samples may undergo a preliminary purification step by liquid-liquid extraction or SPE (Solid Phase Extraction) in order to eliminate interferences and optimize detection sensitivity.

Relevant matrices and industrial applications

This service applies to a wide variety of matrices:

• Dry or hydroalcoholic plant extracts derived from valerian or other relaxing plants (e.g. Centranthus ruber );
• Nutraceutical finished products: capsules, tablets, syrups, infusions, powders or concentrated liquids;
• Raw plant materials intended for industrial processing;
• Ingredients for functional use incorporated into formulations aimed at well-being or sleep.

Valepotriates are essential analytical markers for quality control of valerian-based products, ensuring batch consistency, dosage conformity and formulation stability.

Technical characteristics and quality of results

The assays are performed according to validated protocols, guaranteeing:

• Extraction under an inert atmosphere to preserve the valepotriates;
• The use of certified reference standards (purified valtrate);
• Precise calibrations for reliable quantification, expressed in mg/g of dry extract or as a percentage of dry matter;
• Analytical reproducibility in accordance with the requirements of ISO 17025 .

YesWeLab relies on COFRAC accredited partner laboratories , specializing in the analytical chemistry of plant compounds and the standardization of botanical extracts .

YesWeLab Expertise and Support

YesWeLab supports manufacturers, formulators, and distributors of natural products in the analysis and quality control of their plant extracts. Through an intuitive digital platform , you can easily submit your samples, track the progress of your analyses, and receive your results securely.

Our scientific team helps you define the most suitable analytical protocol (HPLC or LC-MS) according to the matrix, the expected concentration and your certification needs.

Further analyses recommended

For a complete phytochemical characterization of medicinal plant extracts, YesWeLab also recommends:

• Total flavonoids assay
• Determination of phenolic acids
• Determination of volatile compounds by GC-MS
• Trigonelline dosage

These additional analyses make it possible to draw up a complete phytochemical profile of the extracts, to evaluate the synergies between families of molecules and to strengthen the traceability of plant raw materials.

Contact YesWeLab today to schedule your valepotriate (valtrate) assay or to obtain a personalized quote. Our experts will guide you in selecting the analytical methods best suited to your samples and quality objectives.