Thyroxine dosage (total T4 or T4T)

Thyroxine (T4) is one of the main hormones secreted by the thyroid gland. Its measurement, particularly in its total form (T4T), is a key indicator of thyroid activity in animals. In animal health, this analysis helps identify hormonal imbalances related to conditions such as hypothyroidism or hyperthyroidism, and also allows for monitoring the effects of fortified diets or functional nutritional supplements.

T4 analysis is also used in animal nutrition studies, the development of new veterinary products, or breeding programs.

Analytical methods: immunoassay or LC-MS/MS spectrometry

thyroxine levels are measured using two main approaches:

• LC-MS/MS (liquid chromatography coupled with tandem mass spectrometry): this method offers high sensitivity, increased specificity and allows precise quantification even at very low concentrations.
• Immuno-enzymatic method (ELISA or similar): it can be used for routine analyses on standard matrices, when sensitivity requirements are lower.

Analyzed matrices

The method is applicable to a wide variety of matrices from the veterinary and nutritional sectors, such as:

• Animal serums
• Plasmas or biological extracts
• Animal food supplements
• Fortified foods

These matrices can relate to various species (dogs, cats, cattle, horses, etc.) depending on the intended applications.

Industrial Applications and Challenges

The measurement of total thyroxine is relevant in many contexts:

• Endocrine monitoring in pets
• Quality control of veterinary formulations
• Optimization of nutritional supplements enriched with iodine or thyroactive extracts
• Evaluation of biological response in veterinary clinical trials
• Monitoring of side effects in drug treatments

Our analytical guarantees

At YesWeLab, we use a network of expert and rigorously selected partner laboratories, the majority of which are ISO 17025 certified and/or COFRAC accredited. For each project, we select the most suitable laboratory based on:

• From the matrix to analyze
• Necessary analytical techniques
• Sensitivity or regulatory standards to be respected

Thanks to our digital platform, you can centralize, track and simplify your requests for thyroxine analysis and many other veterinary biomarkers.

Other useful additional analyses

For professionals in the veterinary sector, several additional analyses are often requested in parallel with the T4 measurement:

• Calprotectin assay : for monitoring inflammatory conditions
• Lactate dehydrogenase (LDH) assay : to assess cell integrity
• Transferrin loading capacity (TIBC) measurement : an indicator of iron status
• Biomarker assays

You can also consult our complete catalogue of animal health analyses to explore all our solutions.

Entrust your thyroid analyses to YesWeLab

Since 2020, YesWeLab has been supporting manufacturers, distributors, veterinarians, and analytical laboratories in the outsourced management of their analytical needs. Our approach guarantees you:

• A single point of contact
• Prompt handling of your specific requests
• Reliable, traceable results that comply with current standards

Contact our scientific team now to obtain a personalized quote or to discuss your technical specifications.