Dosage of theaflavins

Theaflavins polyphenols formed during the fermentation of black tea. They result from the oxidation of catechins present in tea leaves and are responsible for the amber color as well as certain organoleptic properties characteristic of this beverage. Beyond their sensory role, they have documented antioxidant, cardiovascular, and anti-inflammatory effects. Theaflavin assay is therefore essential for standardizing black tea extracts, controlling the quality of nutraceutical formulations, and ensuring the valorization of these bioactive compounds.

Analytical methods for the determination of theaflavins

The dosage of theaflavins is based on high-precision chromatography techniques:

• HPLC-DAD (high performance liquid chromatography with diode array detection), allowing the separation and quantification of the main theaflavins.
• LC-MS (liquid chromatography coupled with mass spectrometry), used for fine characterization and highly sensitive detection, including in complex matrices.

These analytical approaches are in line with methods applied to the determination of catechins or the determination of flavonoids , also essential for the characterization of tea extracts and other plants rich in polyphenols.

Industrial and sectoral applications

Theaflavin dosage is essential in several areas:

• Human food: quality control of black teas, functional drinks and food products enriched with polyphenols.
• Nutraceuticals: formulation and standardization of food supplements promoting the antioxidant and protective effects of black tea.
• Cosmetics: incorporation of extracts rich in theaflavins in treatments for their protective properties against oxidative stress.
• Scientific research: study of the physiological effects and characterization of the bioactive mechanisms linked to the consumption of black tea.

These applications demonstrate the importance of this analysis for the industrial valorization of tea polyphenols.

Matrices analyzed for theaflavins

YesWeLab supports the analysis of theaflavins in a wide range of matrices: black tea leaves, dry extracts, plant powders, functional drinks, nutraceutical capsules, and cosmetic formulations. Each matrix benefits from a specific preparation to ensure the reliability and reproducibility of results.

Quality and compliance issues

Quantification of theaflavins makes it possible to address several strategic challenges:

• Standardization of black tea extracts to ensure consistent polyphenol content.
• Quality control and authenticity of finished products.
• Highlighting health and nutritional claims related to antioxidants.
• Compliance with safety and traceability standards imposed by regulatory authorities.

These issues are similar to those encountered in other polyphenolic analyses such as the dosage of anthocyanins or the dosage of tannins .

YesWeLab's expertise in theaflavin dosage

YesWeLab relies on a network of more than 200 partner laboratories in France and Europe, accredited ISO 17025 and COFRAC. Our experts select the most appropriate analytical methods based on your objectives: quality control, regulatory compliance, marketing development, or research. Thanks to our digital platform, you can manage your analyses with ease: online quotes, sample tracking, and rapid results delivery. Since 2020, many manufacturers and distributors have entrusted us with their analyses to guarantee the traceability and quality of their tea extracts and enriched products.

Take action

Do you want to control the theaflavin content of your black tea extracts, beverages, or dietary supplements? Entrust your samples to YesWeLab for reliable, standards-compliant results. Request a quote online today.