Tetrahydrocurcumin (curcuminoid) dosage

Tetrahydrocurcumin measurement quantifies this active metabolite of curcumin , known for its superior bioavailability and enhanced antioxidant and anti-inflammatory properties . Produced by the enzymatic biotransformation of curcumin, tetrahydrocurcumin is now a key marker for evaluating the efficacy and stability of turmeric-based nutraceutical formulations .

This analysis is particularly used for quality control of food supplements, standardized extracts and encapsulated formulations . It is performed by high-performance liquid chromatography (HPLC) or by liquid chromatography coupled with mass spectrometry (LC-MS/MS), offering high precision and high sensitivity.

Principle and operation of the method

The samples (turmeric extracts, capsules, powders, softgels or liquid formulations) are subjected to selective extraction of curcuminoids using suitable solvents.

• In HPLC-DAD , separation is based on the polarity and size of the molecules, with detection at 280–425 nm for curcumin derivatives.
• In LC-MS/MS , tetrahydrocurcumin is identified according to its mass/charge ratio (m/z) and quantified with increased accuracy, even at very low concentrations.

These methods guarantee specific identification of tetrahydrocurcumin, including in complex or multicomponent matrices, and allow for the evaluation of the metabolic conversion of curcumin in bioavailability studies.

Analytical performance and related analyses

HPLC and LC-MS methods offer:

• high sensitivity (detection down to a few ng/mL),
• a high specificity allowing curcuminoids to be distinguished from one another
• excellent reproducibility for quality control and batch validation.

For a complete characterization of turmeric-based formulations, this analysis can be combined with:

• the dosage of curcumin,
• the dosage of demethoxycurcumin,
• the dosage of bisdemethoxycurcumin,
• or the measurement of total polyphenols

These additional analyses make it possible to establish the complete profile of curcuminoids and to control the quality of standardized plant extracts.

Applications and areas of use

Tetrahydrocurcumin assays are applicable in various contexts:

• Nutraceuticals : verification of the actual tetrahydrocurcumin content in standardized turmeric supplements and extracts.
• Research and development : studies of metabolic transformation and bioavailability in vitro or in vivo.
• Phytotherapy : evaluation of the quality and purity of active ingredients in natural products.
• Quality control : validation of the composition of curcuminoid-enriched formulations to guarantee their stability and effectiveness.

This analysis thus makes it possible to secure turmeric-based products and to highlight their antioxidant and anti-inflammatory effectiveness.

The advantages of the YesWeLab service

YesWeLab collaborates with a network of ISO 17025 and COFRAC accredited laboratories, experts in the analysis of curcuminoids and plant bioactive compounds. Each project is assigned to the most suitable laboratory based on the matrix, method, and level of precision required.

Thanks to its digital analysis platform, YesWeLab simplifies the management of your analytical services: quote requests, sample tracking, and rapid results delivery. Since 2020, numerous nutraceutical and R&D companies have trusted us for the standardization and quality control of their natural extracts.

For any specific tetrahydrocurcumin dosage requirements, contact our scientific team to obtain a personalized quote and technical support tailored to your needs.