Sequiterpene lactone (parthenolide) determination

Parthenolide is a sesquiterpene lactone naturally present in feverfew ( Tanacetum parthenium ). This bioactive molecule is known for its anti-inflammatory, antioxidant, and anti-migraine properties. It acts on NF-κB signaling pathways and modulates the immune response, making it an essential quality marker for plant extracts used in nutraceuticals and phytotherapy.

Parthenolide analysis allows for the standardization of natural extracts based on feverfew and ensures the conformity of formulations for joint or anti-inflammatory use. This analysis guarantees the traceability, stability, and performance of the finished products.

Objectives of the analysis

The analysis of parthenolide aims to:

• Quantify the sesquiterpene lactone content in extracts of Tanacetum parthenium .
• Verify compliance with quality specifications and target content of active ingredients.
• Evaluate the stability of the compound during storage and processing.
• Control the standardization of plant-based raw materials used in supplements.
• Identify the presence of other related lactones for phytochemical studies.

Analytical methods

Parthenolide assay relies primarily on liquid chromatography, which is suitable for the detection and quantification of nonpolar and thermolabile compounds.

High-performance liquid chromatography (HPLC-DAD)
HPLC coupled with a diode array detector allows the quantification of parthenolide and other sesquiterpene lactones from their UV absorption spectrum. This method is widely used in the quality control of standardized extracts.

Liquid chromatography coupled with mass spectrometry (LC-MS or LC-MS/MS)
offers increased sensitivity and selectivity for the detection of lactones present at very low concentrations. It is preferred for complex matrices (supplements, vegetable oils, multi-plant extracts) and for the structural confirmation of parthenolide.

Substance analyzed

• Parthenolide (CAS 20554-84-1)

Other related sesquiterpene lactones can also be quantified on request, such as costunolide, santamarin or artemisinin, depending on the plant matrix studied.

Affected matrices

• Extracts of feverfew ( Tanacetum parthenium )
• Powders and capsules of dietary supplements for joint health
• Plant-based ingredients with anti-inflammatory properties
• Hydroalcoholic or glycerinated extracts of medicinal plants
• Standardized nutraceutical or phytotherapeutic products

Industrial applications

• Quality control and validation of batches of natural extracts.
• Evaluation of the stability and purity of plant-based active ingredients.
• Standardization of nutraceutical and phytotherapeutic formulations.
• Traceability and authenticity studies of plant extracts.
• Research and development of natural anti-inflammatory formulations.

Recommended Related Analyses

To complete the phytochemical profile and ensure the overall conformity of the extracts, YesWeLab recommends:
Apigenin-7-O-glucoside and alpha-bisabolol assays – for the analysis of flavones and terpenes in chamomile extracts.
Echinacoside assay – for the quantification of phenolic acids in immunomodulatory extracts.
Ferulic acid assay – for the evaluation of the antioxidant potential of plant extracts.
Aromatic volatile compound assays – to complete the sensory and chemical analysis of medicinal plant oils.

Why choose YesWeLab?

YesWeLab relies on a network of partner laboratories accredited to ISO 17025 and specializing in liquid chromatography, guaranteeing reliable and reproducible quantification of sesquiterpene lactones.

Our scientific team assists manufacturers, distributors and formulators in selecting the analytical methods best suited to their matrices, regulatory requirements and technical specifications of natural products.

Thanks to the YesWeLab digital platform, it is possible to centralize requests for analyses, track samples online and obtain results quickly, while benefiting from personalized scientific support.