Rebaudioside C dosage

Rebaudioside C is a steviol glycoside found in the leaves of Stevia rebaudiana , alongside rebaudioside A and stevioside. Less sweet but more balanced in terms of flavor, it contributes to the characteristic taste profile of stevia extracts.

Analysis of rebaudioside C, performed by HPLC-DAD or LC-MS/MS , allows for the quantification of this natural compound in crude extracts, refined sweeteners, or sweetening formulations. This measurement is essential for standardizing stevia-based products , ensuring their consistent taste , and verifying their regulatory compliance .

Rebaudioside C: a steviol glycoside with a specific sweetening profile

Rebaudioside C belongs to the family of glycosylated diterpenes derived from steviol, responsible for the sweet taste of the Stevia rebaudiana . Its chemical structure, slightly different from that of rebaudioside A , influences its sweetness intensity and aromatic profile.

• Sweetening power : approximately 30 to 50% of that of rebaudioside A.
• Sensory advantage : less bitter and rounder taste, often used to soften mixtures of natural sweeteners.
• Natural occurrence : mainly in varieties of Stevia rebaudiana Bertoni rich in secondary glycosides.

This compound is particularly studied for its technological and organoleptic interest , notably in the formulation of sweeteners with a more balanced taste and in the search for natural sugar substitutes without glycemic effect.

Objectives and benefits of rebaudioside C assay

The rebaudioside C assay aims to:

• to determine the glycosidic composition of stevia extracts,
• standardize the production of natural sweeteners according to their sensory profile,
• verify the purity and conformity of products intended for human consumption,
• to monitor the stability of steviol glycosides during purification processes,
• validate the claims “natural sweetener” and “no added sugar”.

The most frequently analyzed matrices include:

• raw or purified stevia extracts ,
• powders and syrups ,
• drinks and diet products ,
• nutraceutical formulations and food supplements.

Analytical methods used

Rebaudioside C is analyzed by HPLC-DAD or LC-MS/MS , two reference methods for the characterization of steviol glycosides.

• HPLC-DAD : the preferred technique for routine assays, allowing the separation of different glycosides on a C18 column and their detection at 210 nm. It offers excellent precision for standardized extracts.
• LC-MS/MS : a more sensitive and specific method, used to confirm the structure of rebaudioside C and to distinguish glycosidic isomers in complex matrices.

The results can be expressed as a percentage of purity or in mg/g of product.

This analysis is often combined with other complementary tests, including:

• Rebaudioside A dosage,
• Stevioside dosage,
• Total steviol glycoside assay,
• Glucose and fructose levels are measured to assess the overall composition of sugars and sweeteners.

Industrial and regulatory applications

Rebaudioside C analysis is essential in several contexts:

• Food industry : quality control of stevia extracts used in drinks, desserts, confectionery and low-fat products.
• Nutraceuticals and food supplements : standardization of stevia-based formulations, particularly those claiming a hypoglycemic or metabolic effect.
• Regulation and compliance : verification of compliance with the specifications of Regulation (EU) No 231/2012 relating to steviol glycosides (E960) and the Codex Alimentarius .
• Research and innovation : study of glycosidic profiles to develop new natural sweeteners with better stability and optimized sweet taste.

These analytical controls ensure safety of use , transparency of labeling and control of the organoleptic quality of products containing stevia extracts.

Carrying out analyses at YesWeLab

YesWeLab relies on a network of partner laboratories specializing in the chromatography of glycosidic compounds high-performance HPLC-DAD and LC-MS/MS instruments

The tests are carried out according to protocols conforming to the ISO 17025 , guaranteeing the reliability, traceability and reproducibility of the results.

The YesWeLab scientific team supports companies in the nutrition, natural ingredients, and food research in:

• the selection of appropriate analytical methods,
• the validation of batches of extracts,
• and the technical interpretation of the results.

Since 2020, YesWeLab has been contributing to the development of natural sweeteners by offering recognized analytical expertise and an intuitive digital platform for the complete management of analyses.

Technical data

• Methods: HPLC-DAD, LC-MS/MS
• Expertise: Physicochemical, nutritional
• Sectors: Nutraceuticals, food processing, natural ingredients, plants
• Measured parameters: Rebaudioside C, total steviol glycosides
• Normative references: ISO 17025, Regulation (EU) No 231/2012, Codex Alimentarius

For any specific request or comparative study between steviol glycosides, contact our scientific team for a personalized quote and tailored technical support.