Rebaudioside A dosage

Rebaudioside A is a steviol glycoside extracted from the leaves of Stevia rebaudiana , a plant native to South America known for its high natural sweetness . This compound, up to 300 times sweeter than sucrose, is used as a low-calorie sweetener in foods, beverages, and nutritional supplements.

Rebaudioside A is measured by HPLC-DAD (high-performance liquid chromatography with UV detection) or LC-MS/MS for precise and selective quantification. This analysis ensures the purity, regulatory compliance, and standardization of stevia-based products intended for the food and nutraceutical industries.

Rebaudioside A: a reference natural sweetener

Rebaudioside A is the main sweetening compound in the Stevia rebaudiana . It belongs to the family of glycosylated diterpenes derived from steviol , which give stevia its characteristic sweet taste without significant calorie content.

Among steviol glycosides, rebaudioside A is one of the purest and most palatable, thanks to its low residual bitterness . It is commonly used as a sugar substitute in:

• non-alcoholic drinks,
• Dairy products and desserts,
• sweets and chewing gum,
• dietary supplements,
• and nutraceutical formulations for metabolic purposes.

Objectives and benefits of rebaudioside A assay

Chromatographic analysis of rebaudioside A allows us to:

• to precisely quantify the steviol glycoside content in stevia extracts,
• verify the regulatory compliance of the sweeteners used (minimum purity, absence of impurities or adulteration),
• evaluate the relative composition between the different glycosides (rebaudiosides A, C, D, F, stevioside),
• to standardize plant extracts intended for food or nutraceutical use,
• validate nutritional claims related to sugar or calorie reduction.

The matrices in question include:

• raw or refined stevia extracts,
• functional or low-fat drinks,
• natural sweeteners (powders, syrups),
• plant-based nutraceutical formulations

Analytical methods used

The measurement of rebaudioside A relies on high-precision chromatographic techniques:

• HPLC-DAD : reference method for the quantification of steviol glycosides in plant extracts and finished products. The analysis is performed on a C18 column, with detection in the UV range around 210 nm.
• LC-MS/MS : method of choice for complex matrices or low concentrations, offering specific identification and high sensitivity.

These analyses also make it possible to distinguish rebaudioside A from other structurally similar molecules such as stevioside , rebaudioside C or dulcoside A , in order to ensure a complete characterization of the extract.

For a complete sweetness profile analysis, this service can be combined with:

• Stevioside dosage,
• Analysis of total steviol glycoside,
• Dosage of glucose and fructose,
• Sucrose dosage.

Industrial and regulatory applications

The dosage of rebaudioside A is part of a strict regulatory framework defined by health authorities (EFSA, FDA, ANSES).

The main applications include:

• Quality control of stevia extracts : verification of purity and steviol glycoside profile in accordance with Codex Alimentarius specifications and Regulation (EU) No 231/2012.
• Formulation of natural sweeteners : development of optimized sweetening mixtures combining rebaudioside A with other natural molecules (erythritol, inulin, mogroside glycosides, etc.).
• Dietary and sugar-free products : control of reduced-fat formulations intended for diabetics or those on a calorie-controlled diet.
• Nutraceutical research and innovation : stability studies, interactions with other ingredients and bioavailability of steviol glycosides.

These analyses guarantee the safety of use , traceability and conformity of products containing stevia, by ensuring accurate labeling of active concentrations.

Carrying out analyses at YesWeLab

YesWeLab collaborates with expert laboratories in the analytical chemistry of natural glycosides HPLC-DAD and LC-MS/MS systems ISO 17025 standards .

The tests are performed according to validated protocols, adapted to the matrix and the expected concentration. The reports include:

• the rebaudioside A content ,
• the overall profile of steviol glycosides ,
• and interpretive comments for regulatory compliance.

Since 2020, YesWeLab has been supporting manufacturers of nutrition, functional beverages and natural ingredients in the analytical control of their sweetening products.

Thanks to its digital platform , YesWeLab simplifies the ordering, tracking and receipt of analyses, while guaranteeing the quality and responsiveness of the service.

Technical data

• Methods: HPLC-DAD, LC-MS/MS
• Expertise: Physicochemical, nutritional
• Sectors: Nutraceuticals, food processing, plants, natural ingredients
• Measured parameters: Rebaudioside A, steviol glycosides
• Normative references: ISO 17025, Codex Alimentarius, Regulation (EU) No 231/2012

To obtain a personalized quote or technical support on the compliance of your natural sweeteners, contact our scientific team today.