Determination of reactive oxygen metabolites (free radical compounds, d-roms)

The measurement of reactive oxygen species (D-ROMs, for Derivatives of Reactive Oxygen Metabolites ) is an essential analysis for assessing the level of oxidative stress within an organism or biological matrix. These unstable compounds, such as free radicals, are naturally produced during metabolic processes but can accumulate in excess under the influence of various environmental, physiological, or pathological factors. Their measurement is a key indicator of redox balance and overall health status, particularly in animal biology, nutritional, and veterinary studies.

Principle and importance of D-ROM measurement

Reactive oxygen species (ROS) encompass several chemical species capable of oxidizing lipids, proteins, or nucleic acids. These include hydrogen peroxide (H₂O₂), superoxide ions (O₂•−), and hydroxyl radicals (•OH). ROS analysis involves quantifying these oxidizing species or their derivatives to measure the degree of oxidation present in a sample.

This assessment is crucial for understanding oxidative stress phenomena, often implicated in inflammation, metabolic disorders, or cellular degeneration. In animal health, monitoring D-ROMs allows for tracking the impact of heat, nutritional, or infectious stress on the physiological performance and productivity of livestock.

Analytical methods and technical principles

The quantification of reactive oxygen species (ROS) relies on several complementary analytical approaches. Liquid chromatography techniques (HPLC or LC-MS) offer high sensitivity and excellent specificity for identifying and quantifying oxidizing compounds and their derivatives. These methods can be combined with UV-Vis spectroscopy , often used in colorimetric assays. In this type of analysis, a specific reagent interacts with ROS to generate a color that can be measured spectrophotometrically, with the optical intensity being directly proportional to the concentration of oxidizing metabolites.

Standardized colorimetric kits also provide a rapid and reliable method for routine analyses, particularly in the context of physiological monitoring or quality control of biological products.

Matrix and application domains

animal health sector , this analysis is used for:

• Assess the redox state of an organism and detect an oxidative imbalance.
• To measure the effects of nutritional or environmental stress on animal performance.
• Monitor the effectiveness of antioxidant, probiotic or phytogenic treatments.
• Characterize the biological quality of raw materials or finished products.

It also finds applications in animal nutrition , veterinary biology research selection and reproduction programs where resistance to oxidative stress is a parameter of interest.

Technical characteristics and analytical requirements

D-ROM analyses are generally performed at a controlled temperature and require careful sample handling to prevent oxidative degradation prior to measurement. Results can be expressed in Carratelli units (U.CARR), corresponding to the equivalent concentration of hydrogen peroxide.

Depending on the needs, this assay can be supplemented by correlated measurements such as total antioxidant capacity (TAC) assessment, determination of reduced/oxidized glutathione, or enzymatic analysis of superoxide dismutase (SOD) and glutathione peroxidase (GPX). These combined approaches provide a comprehensive view of an organism's oxidative/antioxidant profile.

Analytical support and expertise from YesWeLab

YesWeLab provides veterinary industry professionals and researchers with recognized scientific expertise in oxidative stress analysis. Our partner laboratories are selected according to strict criteria of competence, accreditation (ISO 17025, COFRAC), and sector specialization. Through an innovative digital platform, YesWeLab simplifies the management of your analyses, from sample submission to results receipt, while guaranteeing complete traceability.

Our technical team will assist you in selecting the most suitable method for your matrices and objectives: HPLC analysis, UV-Vis spectroscopy, or LC-MS. If you have specific requirements or sample volumes, our scientific advisors will direct you to the most appropriate laboratory within our network.

For those involved in animal health and nutrition , the measurement of D-ROMs represents a reliable biological indicator to assess the state of oxidative stress, optimize feeding protocols and improve animal welfare.

Contact YesWeLab to schedule your analysis or get a personalized quote. Our team will help you define the analytical strategy best suited to your needs.