Dosage of mogroside V (saponin) from grosvenori

Mogroside V is a triterpene saponin extracted from the fruit of Siraitia grosvenorii, commonly known as luo han guo or monk fruit. This compound is responsible for intense sweetness the fruit's 250 times sweeter than sucrose, yet it is calorie-free and has no impact on blood sugar levels.
Used for centuries in traditional Asian medicine, mogroside V is now a key ingredient in nutraceutical and food formulations designed to reduce sugar content without compromising flavor.

Objective of the mogroside V assay

The mogroside V assay aims to precisely quantify this active molecule in:

• ( Monk fruit extracts Siraitia grosvenorii),
• sweeteners Natural,
• incorporating Food or nutraceutical formulations plant-based sweeteners,
• such Finished products as functional drinks, nutritional bars or slimming supplements.

This analysis ensures product standardization, consistency of sweetening power and compliance with marketing claims (“no added sugar”, “naturally sweetened”).

Analytical methods used

At YesWeLab, mogroside V is quantified by high-performance liquid chromatography (HPLC) or mass spectrometry (LC-MS/MS). These techniques offer excellent specificity and sensitivity for the detection of this complex saponin in various matrices.

The main methods are:

• HPLC-DAD (UV/visible detection) : suitable for standardized extracts or products with high concentrations of mogroside V.
• LC-MS/MS (tandem mass spectrometry-coupled chromatography) : reference method for trace detection, identification of other mogrosides (IIE, III, IV) and confirmation of purity.

The analyses are conducted according to good laboratory practices and ISO 17025 standards, guaranteeing the reliability of the results.

Industrial applications and areas of use

The analysis of mogroside V is applicable to many industrial sectors:

• Nutraceuticals : control of the purity and dosage of natural extracts intended for food supplements.
• Functional nutrition : formulation of products with a low glycemic index, intended for diabetic consumers or those seeking natural sugar-free solutions.
• Medicinal plants : standardization of Siraitia grosvenorii extracts in the context of pharmacological studies.
• Natural cosmetics : integration of mogroside V into antioxidant or moisturizing skincare products for its softening and non-irritating effect.

Regulatory framework

In Europe, mogroside V is recognized as a natural sweetener but is not yet listed among the authorized additives (Regulation (EC) No 1333/2008). However, it is accepted in many countries (United States, Japan, Australia) as a GRAS (Generally Recognized As Safe) ingredient.

Pending full validation by the EFSA, its use in nutraceutical products and botanical extracts is regulated by the Novel Food Act (EU Regulation 2015/2283).
Precise measurement of mogroside V is therefore essential to verify regulatory compliance and ensure transparency of composition.

Matrices involved

• Siraitia grosvenorii (monk fruit) extracts
• Blends of natural sweeteners
• Dietary supplements and sweeteners
• Beverages, bars, syrups, nutraceutical formulations
• Cosmetic products based on plant extracts

Recommended related analyses

For a complete characterization of natural sweetening extracts, YesWeLab recommends combining this test with:

• Assay of steviol glycoside (stevia) : monitoring of another natural sweetener with high sweetening power,
• Ginsenoside Rg1 assay : standardization of plant saponins,
• Trigonelline assay : analysis of bioactive alkaloids present in functional plants,
• Verbascoside assay : measurement of a complementary antioxidant polyphenol in plant extracts.

Why entrust your analysis to YesWeLab?

Since 2020, YesWeLab manufacturers nutraceutical, food, and herbal medicine centralize and secure their analyses through its innovative digital platform.
Our partners, accredited to ISO 17025 and often COFRAC, are selected based on the matrix, methodology, and required precision.

Through personalized scientific support, YesWeLab enables formulators, quality managers, and R&D laboratories to:

• Standardize their plant extracts,
• Verify the conformity of their products,
• Accelerate the development of new natural sweetening formulas.

Need to check the mogroside V content in your monk fruit extracts?
now YesWeLab for a quick quote and analysis compliant with international standards.