Dosage of luteolin-7-O-glucoside

Luteolin-7-O-glucoside is a flavonoid glycoside molecule derived from luteolin. Naturally present in many medicinal plants, this conjugated form is particularly abundant in chamomile, artichoke, and olive leaves. Known for its antioxidant, anti-inflammatory, and protective properties, it is often used as an analytical marker in extracts for nutraceutical or cosmetic applications.

The measurement of luteolin-7-O-glucoside is essential for the standardization of plant extracts, the quality control of raw materials, and the verification of compositions in finished products.

Methodology: reliable analysis by HPLC or LC-MS

The analysis of luteolin-7-O-glucoside is performed by high-performance liquid chromatography (HPLC) or liquid chromatography coupled with mass spectrometry (LC-MS). These methods guarantee accurate quantification, even in complex matrices and at low concentrations.

The analytical steps include:

• Optimized sample preparation depending on the nature of the matrix (infusion, dry extract, liquid, plant powder, etc.)
• Selective chromatographic separation
• Detection by UV-DAD or MS/MS, depending on the required sensitivity and specificity

The methods are validated according to the standards of good analytical practices, in accordance with the requirements of ISO 17025 or the COFRAC standard for physico-chemical analyses.

Affected matrices

The assay of luteolin-7-O-glucoside is applicable to various plant matrices or formulations, including:

• Hydroalcoholic or aqueous extracts of medicinal plants
• Infusions, decoctions, and herbal preparations
• Dietary supplements in the form of capsules, tablets or powders
• Plant-based raw materials intended for cosmetics or nutraceuticals
• Industrial and regulatory applications

Luteolin-7-O-glucoside analysis is recommended for:

• The standardization of extracts of chamomile, artichoke, thyme, rosemary, etc.
• The development of antioxidant products based on natural flavonoids
• Studies on the stability and traceability of plant-based active ingredients
• Control of the conformity of ingredients of natural origin in cosmetic and nutraceutical products

This analysis is also used in comparative studies of bioavailability and biological activity of glycosylated flavonoids vs aglycones.

Internal links to other flavonoid analyses

To complement your analysis, also check out:

• Luteolin assay – to compare free and glycosylated forms
• Luteolin-7-glucuronide assay – for metabolism studies
• Apigenin assay – another flavonoid found in chamomile
• Total flavonoid assay – global method for complex extracts

Why choose YesWeLab for your flavonoid analyses?

YesWeLab relies on a network of accredited partner laboratories (ISO 17025, COFRAC, etc.) selected for their expertise in the analysis of phytochemical compounds. Our scientific team supports you in defining your analytical protocol, optimizing extraction conditions, choosing methods, and interpreting results.

Our digital platform allows you to centralize all your analysis requests, track your samples in real time, and access your reports via a secure space.

Do you need a luteolin-7-O-glucoside assay?

Contact our scientific team now for a personalized study or a quick quote tailored to your matrices and analysis objectives.