Apigenin-7-O-glucoside (flavone) assay

Apigenin-7-O-glucoside is a glycosylated flavonoid, a direct derivative of apigenin, widely found in chamomile (Matricaria chamomilla), medicinal plants, and numerous plant matrices used in nutraceuticals and cosmetics. This molecule is particularly valued for its anti-inflammatory, antioxidant, and soothing properties, making it an essential quality marker in standardized plant extracts.

The dosage of apigenin-7-O-glucoside is essential to guarantee the added value of natural ingredients, control their standardization, verify their stability during the manufacturing process and ensure the conformity of finished products.

Why measure apigenin-7-O-glucoside?

Measuring the concentration of this flavone provides essential information for:

• Standardize plant extracts , particularly extracts of chamomile and plants rich in flavonoids.
• Ensuring the reproducibility of batches is a major challenge for manufacturers of food supplements and natural cosmetics.
• Monitor functional efficacy , as apigenin-7-O-glucoside is associated with calming, antioxidant, and anti-inflammatory activities.
• Monitor the stability of the molecule in liquid or dry formulations.
• Check regulatory compliance , in particular for plant-based ingredients used in the composition of products intended for well-being, skincare or health nutrition.

Analytical techniques used

The analysis of apigenin-7-O-glucoside relies on high-precision chromatographic techniques, adapted to complex matrices:

• HPLC-DAD : a common, sensitive and robust method for quantifying flavone even at low concentrations.
• LC-MS or LC-MS/MS : used for maximum selectivity, especially in the case of complex mixtures of polyphenols or interferences.
• UPLC : for rapid analyses, with better resolution on samples highly concentrated in flavonoids.

These methods ensure excellent specificity and allow for clear differentiation of apigenin-7-O-glucoside from free apigenin or other glycosylated derivatives.

Analyzed matrices

The assay can be performed on a wide range of plant matrices or processed products:

• Natural extracts (liquid or dry)
• Standardized vegetable powders
• Nutraceutical ingredients
• Dietary supplements (capsules, tablets, mixtures)
• Natural cosmetic ingredients
• Macerates and decoctions
• Plant-based formulations rich in flavonoids

Industrial applications

This dosage is particularly relevant for the following sectors:

• Nutraceuticals : validation of active ingredient content in chamomile extracts or multi-plant formulations.
• Cosmetics : control of the efficacy and stability of soothing extracts used in skin care.
• Medicinal plants : standardization of raw materials.
• Premium animal nutrition : enhancement of functional plant extracts (calming agents, antioxidants).
• R&D : studies of bioavailability, non-human metabolism or stability.

Further analyses recommended

For a more complete characterization of plant extracts and flavonoids, YesWeLab often recommends combining this dosage with:

• Apigenin dosage
• Apigenin-7-O-glucuronide assay
• Total flavonoids assay
• Determination of total polyphenols
• Luteolin and derivative dosage
• Catechin dosage

Why go through YesWeLab?

With YesWeLab, you benefit from:

• From an expert network of certified and/or accredited laboratories (ISO 17025, COFRAC).
• support adapted to each matrix (plants, extracts, supplements).
• Scientific support is needed to define the appropriate method (HPLC, LC-MS/MS, extraction, sample preparation).
• Simple management via our digital platform , which centralizes quotes, sending samples and results.
• High responsiveness , appreciated by manufacturers, distributors and design offices.

For any specific request, our scientific team will assist you in identifying the method best suited to your needs.