Lactate dehydrogenase (LDH) assay

Lactate dehydrogenase (LDH) is a key enzyme in anaerobic metabolism, catalyzing the conversion of lactate to pyruvate. It is present in most tissues and is a marker of cellular integrity: any release of LDH into biological fluids can indicate injury, inflammation, or cytotoxicity.

LDH testing is particularly useful in:

• Monitoring the physiological state of animals
• Assessment of inflammatory or infectious reactions
• In vitro cytotoxicity tests on animal cell cultures
• Characterization of veterinary or nutraceutical products

Analytical methods

LDH measurement is performed using two main approaches:

• Spectrophotometric enzymatic method: measurement of the catalytic activity of LDH on NADH, rapid and standardized method.
• Enzyme-linked immunosorbent assay (ELISA): specific quantification of LDH isoforms in complex matrices, with high sensitivity.

These methods allow reliable measurement of LDH in different types of samples from animal health.

Frequently analyzed matrices

• Animal serums and plasmas
• Non-medical biological fluids
• Extracts or cell cultures of animal origin
• Enriched or functional veterinary products

Applications of LDH assay

• Quality control of veterinary supplements
• Biocompatibility or cellular toxicity tests
• Animal nutritional or metabolic studies
• Monitoring inflammation markers in veterinary research
• Analysis of the effectiveness of additives or functional ingredients

Why go through YesWeLab?

YesWeLab connects you with a network of laboratories specializing in veterinary biochemical analyses, chosen for their expertise, responsiveness and regulatory compliance (ISO 17025, COFRAC, etc.).

We support you in:

• Choosing the most relevant method
• Interpretation of results in an application context
• Simplified analysis management via our digital platform

Since 2020, many animal health, nutrition and research professionals have trusted us to outsource their analyses.

Recommended internal links

• Calprotectin (inflammation) analysis

Expertise at the service of your studies

Do you want to evaluate a biomarker, validate an ingredient, or verify a physiological effect in animals? Our scientific team will assist you with method selection, sample preparation, and laboratory selection.

Contact us to obtain a personalized quote or to discuss your experimental protocol.