Lactate dehydrogenase (LDH) assay

Lactate dehydrogenase (LDH) is a key enzyme in anaerobic metabolism, catalyzing the conversion of lactate to pyruvate. It is present in most tissues and serves as a marker of cellular integrity: any release of LDH into biological fluids may indicate injury, inflammation, or cytotoxicity.

Measuring LDH levels is particularly useful in:

• Monitoring the physiological state of animals
• The assessment of inflammatory or infectious reactions
• In vitro cytotoxicity tests on animal cell cultures
• Characterization of veterinary or nutraceutical products

Analytical methods

LDH levels are measured using two main approaches:

• Spectrophotometric enzymatic method: measurement of the catalytic activity of LDH on NADH, rapid and standardized method.
• Immunoenzymatic method (ELISA): specific quantification of LDH isoforms in complex matrices, with high sensitivity.

These methods allow for reliable measurement of LDH in different types of samples from animal health.

Frequently analyzed matrices

• Animal serums and plasmas
• Non-medical biological fluids
• Extracts or cell cultures of animal origin
• Enriched or functional veterinary products

Applications of LDH dosage

• Quality control of veterinary supplements
• Biocompatibility or cellular toxicity tests
• Animal nutritional or metabolic studies
• Monitoring inflammation markers in veterinary research
• Analysis of the effectiveness of additives or functional ingredients

Why go through YesWeLab?

YesWeLab connects you with a network of laboratories specializing in veterinary biochemical analyses, chosen for their expertise, responsiveness and regulatory compliance (ISO 17025, COFRAC, etc.).

We support you in:

• Choosing the most relevant method
• Interpreting the results in an application context
• Simplified analysis management via our digital platform

Since 2020, many professionals in animal health, nutrition and research have trusted us to outsource their analyses.

Recommended internal links

• Calprotectin analysis (inflammation)

Expertise at the service of your studies

Do you want to evaluate a biomarker, validate an ingredient or verify a physiological effect in animals? Our scientific team will assist you in selecting the method, preparing samples and choosing laboratories.

Contact us for a personalized quote or to discuss your experimental protocol.