Dosage of iron-oxidizing bacteria

The measurement of iron-oxidizing bacteria allows for the detection and quantification of microorganisms involved in the transformation of ferrous iron (Fe²⁺) into ferric iron (Fe³⁺). These bacteria, present in soils, water, and biofilms, play a major role in corrosion, biofouling, and the degradation of metallic materials. Their identification is essential for preventing damage in industrial circuits, hydraulic infrastructure, and storage systems.

The analysis relies on traditional and molecular microbiological methods, including enumeration on specific culture media, quantitative PCR and DNA sequencing (NGS), ensuring rapid and reliable detection of microbial populations responsible for iron oxidation.

Principle and operation of the method

The sample (water, soil, biofilm, or biological extract) is cultured on a selective medium that promotes the growth of iron-oxidizing bacteria, such as Gallionella , Leptothrix , or Thiobacillus ferrooxidans . Enumeration is performed after incubation, allowing for an estimation of the bacterial load.
In addition, PCR and next-generation sequencing (NGS) enable precise identification of the strains present and the study of microbial diversity associated with corrosion phenomena.

These combined approaches offer a comprehensive view, both quantitative and qualitative, of the microorganisms involved in iron oxidation processes.

Analytical performance and related analyses

Microbiological analyses applied to industrial materials and environments require high sensitivity and methodological rigor. The techniques used make it possible to detect low bacterial concentrations and to assess oxidative activity on iron.

This dosage can be complemented by other services from the YesWeLab catalogue, such as:

• the measurement of sulfate-reducing bacteria, often involved in biogenic corrosion,
• microbiological analysis of biofilms,
• or the measurement of dissolved and oxidized metals to correlate bacterial presence with the chemical evolution of iron.

These cross-analyses make it possible to establish a complete diagnosis of the corrosion or biodeterioration phenomena observed in industrial environments.

Applications and areas of use

The measurement of iron-oxidizing bacteria is used in various technical sectors:

• Materials and polymers : monitoring the microbiological degradation of metals, alloys and polymer coatings subjected to humid conditions.
• Metal packaging : control of internal corrosion and prevention of contamination during the storage of liquid or semi-liquid products.
• Industrial environment : microbiological monitoring of water networks, cooling towers, tanks and closed circuits to anticipate bio-fouling problems.
• Studies of soils and natural waters : identification of bacterial communities involved in the iron cycle and assessment of their impact on environmental quality.

These applications are part of a comprehensive approach to managing corrosion and the durability of materials, in conjunction with other physico-chemical analyses such as FTIR spectroscopy or scanning electron microscopy (SEM).

The advantages of the YesWeLab service

YesWeLab works with a network of partner laboratories specializing in microbiology and materials analysis, all selected according to strict quality criteria and most of which are ISO 17025 certified and COFRAC accredited. Each project is assigned to a laboratory suited to the matrix being studied and the most appropriate method.

Thanks to its digital analysis platform, YesWeLab simplifies the ordering, tracking, and receipt of results. Since 2020, numerous manufacturers, engineering firms, and infrastructure managers have relied on our expertise to prevent microbiological risks related to corrosion and material contamination.

For any specific needs regarding the measurement of iron-oxidizing bacteria, contact our scientific team for personalized support and a customized quote.