Agnuside (iridoid) dosage

Agnuside is a glycosylated iridoid naturally present in the fruits of the chaste tree (Vitex agnus-castus), a medicinal plant well known for its regulating effects on the female hormonal system. This plant active ingredient is mainly used in food supplements intended to relieve:

• Premenstrual syndrome (PMS);
• Menstrual cycle disorders;
• Symptoms related to menopause;
• Mild hormonal imbalances.

Its mode of action, although complex, involves modulation of dopamine, thus influencing the levels of prolactin and other key hormones of the female cycle.

Why dose agnuside?

The dosage of agnuside is essential for manufacturers of chasteberry supplements. It allows:

• Standardization of dry extracts;
• Quality control of raw materials;
• Regulatory compliance in nutraceutical formulations;
• The assurance of consistent effectiveness from batch to batch.

Agnuside is often considered a quality marker in pharmacopoeial monographs and good manufacturing practice guidelines.

Available analytical methods

At YesWeLab, the dosage of agnuside is carried out using several analytical approaches, depending on the matrix to be tested:

• HPLC-DAD: liquid chromatography method with UV detection, suitable for standardized chaste tree extracts;
• LC-MS/MS: coupled mass spectrometry, ideal for complex or very low concentration matrices;
• HPTLC (high performance thin layer chromatography): rapid sieving method used for production controls.

These methods are validated according to the quality standards required by ISO 17025 standards or current regulatory requirements.

Commonly analyzed matrices

We carry out the analysis of agnuside in numerous galenic forms or industrial extracts:

• Dry extracts of Vitex agnus-castus;
• Chasteberry capsules and tablets;
• Mother tinctures and hydroalcoholic extracts;
• Plant-based food supplements for hormonal purposes.

The analysis can also be adapted for multi-ingredient formulations, in which the identification and quantification of agnuside must be differentiated from that of other phenolic or iridoid compounds.

A strategic analysis for the nutraceutical sector

The dosage of agnuside is part of an approach of transparency and promotion of natural products for hormonal purposes. It reinforces:

• Marketing positioning around proven effectiveness;
• Traceability of plant raw materials;
• Analytical reliability in regulatory dossiers or health claims.

Further analyses recommended

For a complete evaluation of your plant extracts, we recommend combining this test with other targeted dosages:

• Dosage of apigenin – flavonoid with calming effect;
• Trigonelline dosage – neuroactive plant alkaloid;
• Dosage of theanine – amino acid with relaxing effects;
• Assay of antioxidant activity – functional valorization of natural extracts.

These analyses provide a comprehensive analytical approach, essential in the formulation of wellness or women's health supplements.

Have your chaste tree extracts analyzed with YesWeLab

At YesWeLab, we provide you with a network of accredited laboratories to carry out your complex analyses. Thanks to our digital platform, you benefit from:

• Personalized support;
• Access to cutting-edge analytical methods;
• Centralized and seamless management of your samples.

Get a personalized quote

Add this test to your online quote request or contact our scientific team to discuss your specific plant extract needs.