Dosage of gentiopicroside (iridoid glycoside)

Gentiopicroside is an iridoid heteroside naturally present in plants of the genus Gentiana , notably yellow gentian ( Gentiana lutea ) digestive, choleretic and bitter properties . This iridoid is recognized as a quality marker for gentian extracts and other phytotherapeutic preparations.

Gentiopicroside testing is used to quantify the active ingredient content in plant extracts, tinctures, powders, and food supplements.
This analysis is essential for:

• Standardize plant ingredients,
• Ensure consistency of formulations,
• And assess the regulatory compliance of finished products intended for the nutraceutical market.

Analytical methods used

The analysis of gentiopicroside is based on high-precision chromatographic techniques:

• HPLC-DAD (High Performance Liquid Chromatography with Diode Array Detection) : allows fine separation and specific spectral detection of gentiopicroside, while ensuring reliable quantification.
• LC-MS (Liquid Chromatography-Mass Spectrometry) : used to confirm the identity of the molecule by analyzing its mass spectrum, particularly useful in complex matrices.

These methods guarantee excellent sensitivity and reproducibility , while ensuring complete traceability of results according to the requirements of ISO and European Pharmacopoeia standards.

For additional analyses on iridoids, it is possible to combine this dosage with that of swertiamarin, also present in gentian extracts.

Affected matrices

The dosage of gentiopicroside can be applied to many matrices:

• Dry extracts and hydroalcoholic tinctures of gentian.
• Powders of dried roots or aerial parts.
• Food supplements based on bitter plants or digestive complexes.
• Nutraceutical formulations combining gentian with other active ingredients (curcumin, artichoke, fennel, etc.).
• The analysis can also be carried out on finished liquid or solid products in order to verify the stability of the active ingredient during storage or processing.

Industrial and scientific applications

The dosage of gentiopicroside is of major interest in several areas:

• Quality control : validation of the conformity of batches of plant extracts according to the specifications of the pharmacopoeia or customer specifications.
• Product development : adjustment of concentrations to obtain consistent efficacy between batches.
• Research and innovation : study of the phytochemical profiles of gentianaceae or new bitter extracts.
• Certification and traceability : guarantee of the purity and authenticity of plant-based raw materials.

These data are also used to demonstrate the functional value and sensory efficacy (bitterness) of natural formulations.

Regulation and standardization

Within the framework of European regulations on food supplements and herbal products , the quantification of gentiopicroside is an element of proof of conformity.
The molecule is often mentioned in the monographs of the European Pharmacopoeia as a reference substance for gentian.

Mastering your concentration guarantees:

• Product safety ,
• The stability of the formulation over time,
• And the recognition of functional efficiency (bitter and digestive activity).

YesWeLab expertise

YesWeLab relies on a network of ISO 17025 and COFRAC accredited partner laboratories , specializing in phytochemical and chromatographic analyses.

Thanks to the YesWeLab digital platform , you can order your analyses, track your samples and consult your reports online with ease.

For any specific request, contact our scientific team now to define the method best suited to your extracts and standardization needs.