Gentiopicroside (iridoid glycoside) dosage

Gentiopicroside is an iridoid glycoside naturally present in plants of the genus Gentiana , notably yellow gentian ( Gentiana lutea ) , widely used for its digestive, choleretic, and bitter . This iridoid is recognized as a quality marker for gentian extracts and other phytotherapeutic preparations.

Gentiopicroside analysis allows for the quantification of the active ingredient content in plant extracts, tinctures, powders, and dietary supplements.
This analysis is essential for:

• Standardize plant-based ingredients,
• Ensuring consistency in formulations,
• And to assess the regulatory compliance of finished products intended for the nutraceutical market.

Analytical methods used

The analysis of gentiopicroside relies on high-precision chromatographic techniques:

• HPLC-DAD (High Performance Liquid Chromatography with Diode Array Detection) : enables fine separation and specific spectral detection of gentiopicroside, while ensuring reliable quantification.
• LC-MS (Liquid Chromatography Coupled with Mass Spectrometry) : used to confirm the identity of the molecule by analyzing its mass spectrum, particularly useful in complex matrices.

These methods guarantee excellent sensitivity and reproducibility , while ensuring complete traceability of results according to the requirements of ISO standards and European pharmacopoeias.

For further analysis of iridoids, it is possible to combine this measurement with that of swertiamarin, which is also present in gentian extracts.

Matrices involved

The assay of gentiopicroside can be applied to many matrices:

• Dry extracts and hydroalcoholic tinctures of gentian.
• Powders of dried root or aerial parts.
• Dietary supplements based on bitter herbs or digestive complexes.
• Nutraceutical formulations combining gentian with other active ingredients (curcumin, artichoke, fennel, etc.).
• The analysis can also be carried out on finished liquid or solid products in order to verify the stability of the asset during storage or processing.

Industrial and scientific applications

The measurement of gentiopicroside is of major interest in several areas:

• Quality control : validation of the conformity of batches of plant extracts according to the specifications of the pharmacopoeia or the customer's specifications.
• Product development : adjusting concentrations to achieve consistent effectiveness across batches.
• Research and innovation : study of the phytochemical profiles of gentianaceae or new bitter extracts.
• Certification and traceability : guarantee of the purity and authenticity of plant-based raw materials.

This data is also used to demonstrate the functional value and sensory efficacy (bitterness) of natural formulations.

Regulation and standardization

Under European regulations on food supplements and herbal products , the quantification of gentiopicroside is a means of demonstrating compliance.
The molecule is frequently mentioned in the European Pharmacopoeia as a reference substance for gentian.

Mastering one's concentration guarantees:

• The safety of use of the product,
• The stability of the formulation over time,
• And recognition of functional efficacy (bitter and digestive activity).

YesWeLab's expertise

YesWeLab relies on a network of partner laboratories accredited to ISO 17025 and COFRAC , specializing in phytochemical and chromatographic analyses.

Thanks to the YesWeLab digital platform , you can easily order your analyses, track your samples and view your reports online

For any specific request, contact our scientific team now to define the most suitable method for your extracts and standardization needs.