Delphinidin-3-O-sambubioside dosage

Delphinidin-3-O-sambubioside is a specific anthocyanin belonging to the flavonoid family, found primarily in black elderberry ( Sambucus nigra ), but also in certain plants rich in natural pigments. This compound is responsible for part of the characteristic violet to dark blue coloration of these plant matrices. It is now recognized for its marked antioxidant properties, its protective potential against oxidative stress, and its biological effects studied in nutraceutical, functional, and cosmetic applications. In an industrial context, the precise measurement of delphinidin-3-O-sambubioside is essential to guarantee the quality, standardization, and conformity of plant extracts and finished products.

Analytical and industrial interest of the dosage

Delphinidin-3-O-sambubioside assay addresses several key challenges for manufacturers in the plant and nutraceutical sectors. First, it allows for the standardization of plant extracts , ensuring a consistent active ingredient content from batch to batch. This consistency is essential for guaranteeing the functional efficacy of products, as well as for ensuring the validity of nutritional or wellness claims associated with anthocyanins. The assay is also used for quality control to verify the conformity of raw materials, concentrated extracts, juices, and dietary supplements. In R&D, it is a key tool for comparing different extraction methods, optimizing processes, and evaluating the impact of storage and aging on anthocyanin stability.

Principle and operation of the HPLC method

The analysis of delphinidin-3-O-sambubioside relies on high-performance liquid chromatography (HPLC or UPLC), the reference technique for the separation and quantification of phenolic compounds. After sample preparation (extraction, filtration, and possible dilution), the sample is injected into a chromatographic system equipped with a column suitable for anthocyanins. Separation is based on the interactions between the stationary phase and the compounds present in the matrix.
Detection is generally performed by UV-visible spectroscopy , exploiting the characteristic absorption spectrum of anthocyanins, or by mass spectrometry (LC-MS) when analytical specificity needs to be enhanced. The use of a reference standard allows for reliable and reproducible quantification of delphinidin-3-O-sambubioside, even in complex matrices containing numerous structurally similar anthocyanins. This approach ensures excellent sensitivity and high selectivity.

Applications in the nutraceutical and plant sectors

In the nutraceutical sector, the measurement of delphinidin-3-O-sambubioside is particularly relevant for dietary supplements based on elderberry, berry extracts, or antioxidant formulations. It allows for validation of the active ingredient content, ensures batch consistency, and meets traceability and quality requirements.
In the field of plants and plant extracts, this analysis is used to characterize raw materials, compare different botanical origins, or evaluate the pigment content of an extract. It can also be used in stability studies aimed at understanding the evolution of anthocyanins under the influence of light, temperature, or pH. In cosmetics, this measurement can contribute to the validation of naturally derived active ingredients incorporated into protective or antioxidant formulations.

Analyzed matrices

Delphinidin-3-O-sambubioside can be measured in a wide variety of matrices: fresh or dried berries, plant powders, liquid or dry extracts, juices, concentrates, dietary supplements in capsule, tablet, or solution form, as well as functional ingredients intended for the nutraceutical or cosmetic industries. Analytical methods are adapted to the complexity of each matrix to ensure reliable and interpretable results.

Quality framework and reliability of results

Analyses are performed according to validated protocols, using high-performance chromatographic equipment and appropriate reference standards. The results obtained can be directly used for quality control, R&D, or in technical documentation for partners or regulatory authorities. Traceability of analyses and reproducibility of measurements are essential criteria for manufacturers requiring a high level of reliability.

Added value YesWeLab

YesWeLab relies on a network of expert laboratories specializing in the chromatographic analysis of plant and nutraceutical compounds. The majority of our partners are ISO 17025 and COFRAC certified or accredited, guaranteeing the quality and compliance of results. Our scientific team assists you in selecting the analytical method best suited to your matrix, your objective (standardization, quality control, R&D), and your regulatory requirements. Since 2020, manufacturers, distributors, and engineering firms have entrusted us with the management of their analyses via our digital platform, ensuring centralized, rigorous, and responsive monitoring.

Related analyses in the YesWeLab catalog

To complement the measurement of delphinidin-3-O-sambubioside, the following tests are frequently performed:

• HPLC profile of polyphenols and flavonoids
• Assay of cyanidin-3-O-glucoside and other anthocyanins
• Total polyphenol content
• Stability studies of plant extracts