Cyanidin-3-O-glucoside (anthocyanin) dosage

Cyanidin-3-O-glucoside is a water-soluble anthocyanin widely found in colorful plants, particularly red fruits such as blueberries, blackberries, cherries, and grapes, as well as in certain pigmented vegetables. This natural compound belongs to the large family of flavonoids and is distinguished by its intense color and numerous health benefits. It is notably recognized for its antioxidant, anti-inflammatory, vasculoprotective, and neuroprotective effects.

Why measure cyanidin-3-O-glucoside?

Measuring cyanidin-3-O-glucoside levels is essential for several reasons:

• Quality control: it allows verification of the concentration of anthocyanin in plant extracts, juices, powders or food supplements.
• Product standardization: it is essential to guarantee the reproducibility of batches in nutraceutical or cosmetic formulations.
• Claim validation: the precise quantification of this active ingredient makes it possible to support functional (antioxidant, protective…) or coloring claims.
• Traceability and stability: it is used to track color degradation in agri-food products, particularly in natural formulations.

Analytical Methodology

The test relies on chromatographic analysis, using HPLC-DAD (high-performance liquid chromatography with UV-visible detection) or LC-MS/MS , depending on the sensitivity and selectivity requirements. The sample is first prepared by hydroalcoholic extraction, then injected into a chromatographic system equipped with a column suitable for anthocyanins (most often the C18 phase).

Detection is done at specific wavelengths (510-530 nm) or by mass spectrometry for molecular confirmation.

Matrices involved

• Extracts of red fruits or berries (blueberry, blackcurrant, aronia, etc.)
• Fortified juices and drinks
• Dietary supplements based on natural pigments
• Plant powders
• Cosmetic ingredients derived from colored plants

Application sectors

• Nutraceuticals : Dosage for antioxidant active ingredients
• Food processing : natural colorings, beverages, confectionery, sauces
• Cosmetics : tinted or natural protective formulations
• Research : studies of bioavailability or pigment stability

Further analyses recommended

For a more complete characterization of your extracts, we recommend:

• Total anthocyanin assay : to evaluate all the pigments present in an extract.
• Cyanidin dosage : related anthocyanin, useful for mixed extracts.
• Flavonoid dosage : for a broadened antioxidant profile.
• Coumarin dosage : analysis of other natural aromatic compounds.

Why use YesWeLab?

YesWeLab provides you with a network of partner laboratories specializing in high-precision chromatographic assays. Our laboratories are selected for their expertise in HPLC and LC-MS/MS and hold the necessary certifications (ISO 17025, COFRAC). We support you in selecting the most appropriate method for your matrix, performing the tests, and interpreting the results.

Our digital platform allows you to centralize your analysis requests, track your samples and download your reports with ease.

Contact our scientific team to obtain a quote or ask your questions.