Cyanidin-3-O-glucoside (anthocyanin) dosage

Cyanidin-3-O-glucoside is a water-soluble anthocyanin found widely in colorful plants, particularly in red fruits such as blueberries, blackberries, cherries, grapes, and certain pigmented vegetables. This natural compound belongs to the large family of flavonoids and is distinguished by its intense color and multiple health benefits. It is particularly recognized for its antioxidant, anti-inflammatory, vasculoprotective, and neuroprotective effects.

Why measure cyanidin-3-O-glucoside?

The dosage of cyanidin-3-O-glucoside is essential for several reasons:

• Quality control: it allows the concentration of anthocyanin in plant extracts, juices, powders or food supplements to be checked.
• Product standardization: it is essential to guarantee the reproducibility of batches in nutraceutical or cosmetic formulations.
• Validation of claims: the precise quantification of this active ingredient makes it possible to support functional (antioxidant, protective, etc.) or coloring claims.
• Traceability and stability: it is used to monitor color degradation in agri-food products, particularly in natural formulations.

Analytical methodology

The test is based on chromatographic analysis, via HPLC-DAD (high performance liquid chromatography with UV-visible detection) or LC-MS/MS , depending on the sensitivity and selectivity requirements. The sample is first prepared by hydroalcoholic extraction, then injected into a chromatographic system equipped with a column suitable for anthocyanins (most often C18 phase).

Detection is done at specific wavelengths (510-530 nm) or by mass spectrometry for molecular confirmation.

Affected matrices

• Extracts of red fruits or berries (blueberry, blackcurrant, aronia, etc.)
• Juices and fortified drinks
• Food supplements based on natural pigments
• Vegetable powders
• Cosmetic ingredients from colorful plants

Sectors of application

• Nutraceutical : dosage for antioxidant active ingredients
• Food industry : natural colorants, drinks, confectionery, sauces
• Cosmetics : tinted or natural protective formulations
• Research : bioavailability or pigment stability studies

Further analyses recommended

For a more complete characterization of your extracts, we recommend:

• Total anthocyanin assay : to assess all the pigments present in an extract.
• Cyanidin assay : related anthocyanin, useful for mixed extracts.
• Flavonoid dosage : for a broader antioxidant profile.
• Coumarin assay : analysis of other natural aromatic compounds.

Why use YesWeLab?

YesWeLab offers you a network of partner laboratories specializing in high-precision chromatographic assays. Our laboratories are selected for their expertise in HPLC and LC-MS/MS, and hold the necessary certifications (ISO 17025, COFRAC). We assist you in selecting the most appropriate method for your matrix, performing tests, and interpreting results.

Our digital platform allows you to centralize your analysis requests, track your samples and download your reports with ease.

Contact our scientific team to obtain a quote or ask your questions.