Dosage of centella saponosides (asiaticoside)

Asiaticoside is a triterpene saponin, centella saponosides extracted from Centella asiatica , a traditional medicinal plant widely used in Asia and Africa. This molecule is one of the main quality markers of Centella extracts, renowned for their healing, soothing, antioxidant and anti-aging properties. Thanks to these effects, asiaticoside is highly sought after in cosmetic formulations (repair creams, moisturizing serums, anti-wrinkle treatments) and in nutraceutical products focused on skin well-being and tissue health.

Interest in the dosage of asiaticoside

The quantification of asiaticoside is an essential step for various industrial players:

• In cosmetics and dermatology: validate the actual concentration of the molecule in creams, serums or lotions, in order to ensure demonstrated efficacy and consistency between batches.
• In nutraceuticals: standardize Centella extracts intended for food supplements and guarantee their compliance with health claims.
• In research and development: study the stability of asiaticoside over time or in different formulations.
• In quality control: verify the authenticity of raw materials and avoid the risk of fraud on plant extracts.

Analytical methods

The determination of asiaticoside is based on robust and sensitive chromatographic techniques:

• HPLC-UV or HPLC-DAD : method commonly used to separate and quantify saponins in crude extracts.
• LC-MS/MS : mass spectrometry coupled with liquid chromatography, offering increased specificity and sensitivity, allowing detection even at trace levels in complex matrices such as cosmetic creams or enriched powders.

These approaches guarantee reliable and reproducible analysis, adapted to regulatory and industrial requirements.

Affected matrices

The dosage of asiaticoside is applicable to a wide range of products:

• Centella asiatica extracts (dry or liquid).
• Cosmetics: restorative creams, anti-aging serums, soothing lotions.
• Food supplements: powders, capsules, tablets formulated from Centella.
• Herbal pharmaceutical products: dermatological or healing formulations.

Industrial applications

Asiaticoside analysis has several key applications:

• Quality control of plant raw materials.
• Standardization of Centella extracts to ensure homogeneous batches.
• Support for cosmetic and nutraceutical claims (healing, anti-aging, antioxidant effects).
• Evaluation of the stability of formulations in the context of aging studies.

Related analyses in the YesWeLab catalog

The dosage of asiaticoside is part of a wider range of analyses of saponins and active plant compounds :

• Dosage of protodioscin , a steroidal saponin for nutraceutical purposes.
• Dosage of convallatoxin , a cardiotonic glycoside from lily of the valley.
• Dosage of miliacin, a pentacyclic triterpene marker of certain ancient cereals.
• Dosage of active ingredients , generic analysis allowing the quantification of numerous bioactive molecules in extracts and finished products.

These additional analyses provide a complete overview of the composition of plant extracts and enable their industrial use to be optimized.

YesWeLab Expertise

At YesWeLab, we work with a network of specialized laboratories, certified and accredited according to international standards (ISO 17025, COFRAC). Our scientific team assists you in selecting the analytical method best suited to your needs and in interpreting the results. Thanks to our digital platform , you can centralize your analysis requests, track your samples and retrieve your results with ease.