Dosage of centella saponosides (asiaticoside)

Asiaticoside is a triterpene saponin, a centella saponoside extracted from Centella asiatica , a traditional medicinal plant widely used in Asia and Africa. This molecule is one of the main quality markers of Centella extracts, renowned for their healing, soothing, antioxidant, and anti-aging properties. Thanks to these effects, asiaticoside is highly sought after in cosmetic formulations (repairing creams, hydrating serums, anti-wrinkle treatments) and in nutraceutical products focused on skin health and tissue well-being.

Importance of asiaticoside measurement

Quantifying asiaticoside is a crucial step for various industrial players:

• In cosmetics and dermatology: to validate the actual concentration of the molecule in creams, serums or lotions, in order to ensure proven efficacy and consistency between batches.
• In nutraceuticals: standardize Centella extracts intended for food supplements and guarantee their compliance with health claims.
• In research and development: studying the stability of asiaticoside over time or in different formulations.
• In quality control: verify the authenticity of raw materials and avoid the risks of fraud on plant extracts.

Analytical methods

The assay of asiaticoside relies on robust and sensitive chromatographic techniques:

• HPLC-UV or HPLC-DAD : a method commonly used to separate and quantify saponins in crude extracts.
• LC-MS/MS : mass spectrometry coupled with liquid chromatography, offering increased specificity and sensitivity, allowing detection even at trace levels in complex matrices such as cosmetic creams or enriched powders.

These approaches guarantee a reliable and reproducible analysis, adapted to regulatory and industrial requirements.

Affected matrices

Asiaticoside assay is applicable to a wide range of products:

• Extracts of Centella asiatica (dry or liquid).
• Cosmetics: repairing creams, anti-aging serums, soothing lotions.
• Dietary supplements: powders, capsules, tablets formulated from Centella.
• Plant-based pharmaceutical products: dermatological or healing formulations.

Industrial applications

Asiaticoside analysis has several key applications:

• Quality control of plant-based raw materials.
• Standardization of Centella extracts to guarantee homogeneous batches.
• Support for cosmetic and nutraceutical claims (healing, anti-aging, antioxidant effects).
• Evaluation of stability in the context of aging studies.

Related analyses in the YesWeLab catalog

The measurement of asiaticoside is part of a broader range of analyses of saponins and active plant compounds :

• Assay of protodioscin , a steroidal saponin with nutraceutical applications.
• Measurement of convallatoxin , a cardiotonic glycoside derived from lily of the valley.
• Measurement of miliacin, a pentacyclic triterpene marker of certain ancient cereals.
• Dosage of active ingredients , generic analysis allowing the quantification of numerous bioactive molecules in extracts and finished products.

These additional analyses provide a complete view of the composition of plant extracts and optimize their industrial use.

YesWeLab Expertise

At YesWeLab, we work with a network of specialized laboratories, certified and accredited according to international standards (ISO 17025, COFRAC). Our scientific team assists you in selecting the analytical method best suited to your needs and in interpreting the results. Thanks to our digital platform , you can centralize your analysis requests, track your samples, and easily retrieve your results.